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Gene Expression Data
Assay Details
Assay
Reference: J:289834 Ishiuchi T, et al., Zfp281 Shapes the Transcriptome of Trophoblast Stem Cells and Is Essential for Placental Development. Cell Rep. 2019 May 7;27(6):1742-1754.e6
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6477560
Gene symbol: Zfp281
Gene name: zinc finger protein 281
Antibody: anti-ZNF281 (sc-66933)
Detection system: Secondary antibody
Assay notes: The authors did not specify which antibody was used. It is possible anti-Zfp281 (ab101318) (MGI:6476905) was used.
Results
Specimen 3E E6.5: embryonic day 6.5 (more )
Structure Level Pattern Image Note
TS9: extraembryonic ectoderm Present 3E E6.5 Zfp281 , 3E E6.5 2 , 3E E6.5 merge , 3E E6.5 1
TS9: ectoplacental cone Weak 3E E6.5 merge , 3E E6.5 2 , 3E E6.5 1 , 3E E6.5 Zfp281
Specimen 3E E7.5: embryonic day 7.5 (more )
Structure Level Pattern Image Note
TS11: extraembryonic ectoderm Present 3E E7.5 Merge , 3E E7.5 2 , 3E E7.5 1 , 3E E7.5 Zfp281
TS11: ectoplacental cone Present 3E E7.5 1 , 3E E7.5 Merge , 3E E7.5 2 , 3E E7.5 Zfp281
Specimen S3E: embryonic day 6.5 (more )
Structure Level Pattern Image Note
TS9: primitive endoderm Absent S3E Zfp281 left , S3E Zfp281 Merge right , S3E Zfp281 Merge left , S3E Zfp281 right
Specimen S3F Control: embryonic day 6.5 (more )
Note: Also stained with Phalloidin (green).
Structure Level Pattern Image Note
TS9: extraembryonic ectoderm Present S3F control
TS9: ectoplacental cone Weak S3F control
Specimen S3F Zfp281 KO: embryonic day 6.5; Zfp281em1Hsas/Zfp281em1Hsas (more )
Note: Also stained with Phalloidin (green). The authors stated identical results were obtained with both mutants; both have been annotated.
Structure Level Pattern Image Note
TS9: ectoplacental cone Absent S3F KO
TS9: extraembryonic ectoderm Absent S3F KO
Specimen S3F KO: embryonic day 6.5; Zfp281em2Hsas/Zfp281em2Hsas (more )
Note: The authors stated identical results were obtained with both mutants; both have been annotated.
Structure Level Pattern Image Note
TS9: ectoplacental cone Absent
TS9: extraembryonic ectoderm Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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