Antibody:	TROMA 1
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS20: ureter	Present		7A E12.5 +/+	In wild type the ureter is well-separated from the Wolffian duct.
TS20: nephric duct, metanephric portion	Present		7A E12.5 +/+	Expression is seen in the Wolffian duct.
TS20: common nephric duct	Present		7A E12.5 +/+	In wild type the cnd is short.

Structure	Level	Pattern	Image	Note
TS20: ureter	Present		7A E12.5 Y53ANeo/Y53ANeo	In the mutant there is a flat, triagle-shaped structure connecting the Wolffian duct, ureteric bud and common nephric duct.
TS20: nephric duct, metanephric portion	Present		7A E12.5 Y53ANeo/Y53ANeo	Expression is seen in the Wolffian duct.
TS20: common nephric duct	Present		7A E12.5 Y53ANeo/Y53ANeo	In the mutant the cnd is longer than in wild type.

Structure	Level	Pattern	Image	Note
TS21: ureter	Present		7A E13.5 +/+
TS21: nephric duct	Present		7A E13.5 +/+	Expression is seen in the Wolffian duct. In wild type the cnd is almost eliminated.
TS21: metanephros	Present		7A E13.5 +/+

Structure	Level	Pattern	Image	Note
TS21: metanephros	Present		7A E13.5 Y53ANeo/Y53ANeo , 7B	In the mutant the kidney is misplaced caudally.
TS21: ureter	Present		7B , 7A E13.5 Y53ANeo/Y53ANeo	In the mutant there are multiple ureteric buds connected to the kidneys.
TS21: nephric duct	Present		7B , 7A E13.5 Y53ANeo/Y53ANeo	Expression is seen in the Wolffian duct.

Structure	Level	Pattern	Image	Note
TS23: metanephros	Present		7C +/+ male
TS23: ureter	Present		7C +/+ male
TS23: nephric duct of male	Present		7C +/+ male

Structure	Level	Pattern	Image	Note
TS23: metanephros	Present		7C Y53ANeo/Y53ANeo male
TS23: paramesonephric duct	Present		7C Y53ANeo/Y53ANeo male	There is a megaureter in the mutant; it includes a triagle-shaped structure and an enlarged common nephric duct.

Structure	Level	Pattern	Image	Note
TS23: metanephros	Present		7C +/+ female
TS23: ureter	Present		7C +/+ female , 7E +/+
TS23: nephric duct of female	Present		7E +/+ , 7C +/+ female	Expression is seen in the Wolffian duct.
TS23: paramesonephric duct	Present		7C +/+ female	Expression is seen in the Mullerian duct.

Structure	Level	Pattern	Image	Note
TS23: metanephros	Present		7D , 7C Y53ANeo/Y53ANeo female	There are supernumary kidneys in the mutant.
TS23: ureter	Present		7C Y53ANeo/Y53ANeo female , 7D	There is a megaureter in the mutant; it includes a triagle-shaped structure and an enlarged common nephric duct. There are also supernumerary ureters.
TS23: paramesonephric duct	Present		7E Y53ANeo/Y53ANeo , 7C Y53ANeo/Y53ANeo female	Instead of fusing to each other at the midline, in the mutant the Mullerian ducts are coiled.
TS23: nephric duct of female	Present		7C Y53ANeo/Y53ANeo female , 7E Y53ANeo/Y53ANeo	In the mutant ureter maturation never occurs; the common nephric duct inserts directly into the pelvic urethra.