Gene Expression Data
Assay Details
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Assay
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Reference:
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J:143513
Lathia JD, et al., Toll-like receptor 3 is a negative regulator of embryonic neural progenitor cell proliferation. J Neurosci. 2008 Dec 17;28(51):13978-84
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6476687
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Gene symbol:
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Pcna
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Gene name:
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proliferating cell nuclear antigen
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Antibody:
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anti-PCNA (ab29)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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Total Pcna positive cells per area were quantitated and graphed.
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Results
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Specimen
5A E13.5 Ctrl:
embryonic day 13.5
(more  )
Specimen
5A E13.5 Ctrl:
(close  )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5A E13.5 Trl3-/-:
(close )
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Genetic Background:
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B6.129S1-Tlr3tm1Flv/J
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Mutant Allele(s):
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Tlr3tm1Flv/Tlr3tm1Flv
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: telencephalon |
Strong |
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There was a significant increase in expression in this mutant compared to controls.
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Specimen
5A E16.5 Ctrl:
embryonic day 16.5
(more )
Specimen
5A E16.5 Ctrl:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5A E16.5 Trl3-/-:
(close )
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Genetic Background:
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B6.129S1-Tlr3tm1Flv/J
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Mutant Allele(s):
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Tlr3tm1Flv/Tlr3tm1Flv
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: telencephalon |
Present |
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Expression in this mutant was similar to controls.
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Analysis Tools
