Probe:	Nkx3.1 cDNA4
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS22: lower urinary tract	Absent		2B	There is no expression in the area of the prospective prostate.
TS22: lower urogenital tract epithelium	Absent		2B , 2F
TS22: lower urogenital tract mesenchyme	Absent		2B , 2F

Structure	Level	Pattern	Image	Note
TS23: epithelium of pelvic urethra of male	Present	Regionally restricted	2C	Expression is seen in the lateral urogenital sinus epithelium and is excluded from the dorsal and ventral sides; this is the region of the prospective prostate.
TS23: basal cell layer of pelvic urethra of male	Present	Regionally restricted	2C , 2G	Expression is seen in the lateral urogenital sinus epithelium and is excluded from the dorsal and ventral sides; this is the region of the prospective prostate.
TS23: intermediate cell layer of pelvic urethra of male	Absent		2G , 2C
TS23: superficial cell layer of pelvic urethra of male	Absent		2G , 2C
TS23: mesenchymal layer of pelvic urethra of male	Absent		2G , 2C

Structure	Level	Pattern	Image	Note
TS24: epithelium of pelvic urethra of male	Present	Regionally restricted	2D	Expression is seen in the lateral urogenital sinus epithelium.
TS24: epithelium of anterior prostate bud	Present		2D , 2H
TS24: mesenchymal layer of pelvic urethra of male	Absent		2D , 2H

Structure	Level	Pattern	Image	Note
TS25: epithelium of ventral prostate bud	Present		2E
TS25: epithelium of pelvic urethra of male	Absent		2E	There is no expression in the prospective urethral epithelium.
TS25: epithelium of dorsal prostate bud	Present		2E , 2I
TS25: epithelium of lateral prostate bud	Present		2E , 2I

Structure	Level	Pattern	Image	Note
TS22: lower urogenital tract epithelium	Strong	Regionally restricted	2J	Expression is seen in the region of the prospective bulbourethral gland.

Structure	Level	Pattern	Image	Note
TS23: epithelium of bulbourethral gland of male	Strong		2K

Structure	Level	Pattern	Image	Note
TS24: epithelium of bulbourethral gland of male	Strong		2L	Expression is seen in the nascent bulbourethral gland and the ducts that join them to the prospective urethra.

Structure	Level	Pattern	Image	Note
TS25: epithelium of bulbourethral gland of male	Strong		2M	Expression is seen in the nascent bulbourethral gland and the ducts that join them to the prospective urethra.

Structure	Level	Pattern	Image	Note
TS22: lower urogenital tract	Absent			Expression was not detected in the female urogenital sinus at any stage.

Structure	Level	Pattern	Image	Note
TS23: pelvic urethra of female	Absent			Expression was not detected in the female urogenital sinus at any stage.

Structure	Level	Pattern	Image	Note
TS24: pelvic urethra of female	Absent			Expression was not detected in the female urogenital sinus at any stage.

Structure	Level	Pattern	Image	Note
TS25: pelvic urethra of female	Absent			Expression was not detected in the female urogenital sinus at any stage.

Structure	Level	Pattern	Image	Note
TS27: epithelium of anterior prostate bud	Strong	Non-Uniform	2N	Expression is most intense at the ends of the outgrowing prostatic ducts, corresponding to regions of active morphogenesis.

Structure	Level	Pattern	Image	Note
TS27: epithelium of dorsal prostate bud	Strong	Non-Uniform	2O	Expression is most intense at the ends of the outgrowing prostatic ducts, corresponding to regions of active morphogenesis.
TS27: epithelium of lateral prostate bud	Strong	Non-Uniform	2O	Expression is most intense at the ends of the outgrowing prostatic ducts, corresponding to regions of active morphogenesis.

Structure	Level	Pattern	Image	Note
TS27: epithelium of ventral prostate bud	Strong	Non-Uniform	2P	Expression is most intense at the ends of the outgrowing prostatic ducts.

Structure	Level	Pattern	Image	Note
TS27: epithelium of bulbourethral gland of male	Present	Uniform	2Q

Structure	Level	Pattern	Image	Note
TS27: seminal vesicle	Absent		2R

Structure	Level	Pattern	Image	Note
TS28: anterior prostatic epithelium	Strong	Non-Uniform	2S	Expression is most intense at the ends of the outgrowing prostatic ducts, corresponding to regions of active morphogenesis.

Structure	Level	Pattern	Image	Note
TS28: dorsal prostatic epithelium	Strong	Non-Uniform	2T	Expression is most intense at the ends of the outgrowing prostatic ducts, corresponding to regions of active morphogenesis.
TS28: lateral prostatic epithelium	Strong	Non-Uniform	2T	Expression is most intense at the ends of the outgrowing prostatic ducts, corresponding to regions of active morphogenesis.

Structure	Level	Pattern	Image	Note
TS28: ventral prostatic epithelium	Strong	Non-Uniform	2U	Expression is most intense at the ends of the outgrowing prostatic ducts, corresponding to regions of active morphogenesis.

Structure	Level	Pattern	Image	Note
TS28: epithelium of bulbourethral gland of male	Present	Uniform	2V

Structure	Level	Pattern	Image	Note
TS28: seminal vesicle	Absent		2W

Structure	Level	Pattern	Image	Note
TS28: anterior prostatic epithelium	Strong
TS28: dorsal prostatic epithelium	Strong
TS28: lateral prostatic epithelium	Strong
TS28: ventral prostatic epithelium	Strong