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Gene Expression Data
Assay Details
Assay
Reference: J:173123 Mahoney Rogers AA, et al., Sprouty1 and Sprouty2 limit both the size of the otic placode and hindbrain Wnt8a by antagonizing FGF signaling. Dev Biol. 2011 May 1;353(1):94-104
Assay type: RNA in situ
MGI Accession ID: MGI:6471924
Gene symbol: Spry2
Gene name: sprouty RTK signaling antagonist 2
Probe: Spry2 probe8
Probe preparation: labelled with digoxigenin RNA
Results
Specimen 3E: embryonic day 8.0; Spry1tm1.1Jdli/Spry1+, Spry2tm1.1Mrt/Spry2+ (more )
Note: 5-6 somite stage.
Structure Level Pattern Image Note
TS12: otic pre-placode Present 3E Expression in ectoderm of pre-otic field.
TS12: head mesenchyme Present Regionally restricted 3E Expression in layer of mesenchymal cells just underlying the ectoderm.
TS12: branchial arch endoderm Present 3E Expression in pharyngeal endoderm and adjacent pharyngeal mesenchyme
TS12: branchial arch mesenchyme Present 3E Expression in pharyngeal endoderm and adjacent pharyngeal mesenchyme
TS12: embryo endoderm Present Regionally restricted 3E Expression in underlying ventral endoderm.
TS12: future hindbrain Present Regionally restricted 3E Expression in dorsal region of hindbrain starting at pre-otic field stage.
Specimen 3F: embryonic day 8.5; Spry1tm1.1Jdli/Spry1+, Spry2tm1.1Mrt/Spry2+ (more )
Note: 9-10 somite stage.
Structure Level Pattern Image Note
TS13: otic placode epithelium Present 3F Expression in placodal ectoderm.
TS13: otic placode mesenchyme Present Regionally restricted 3F Expression in mesenchyme underlying otic placode.
TS13: head surface ectoderm Present Regionally restricted 3F Expression in surface ectoderm ventral to otic placode.
TS13: branchial arch endoderm Present 3F Expression in pharyngeal endoderm and adjacent pharyngeal mesenchyme
TS13: branchial arch mesenchyme Present 3F Expression in pharyngeal endoderm and adjacent pharyngeal mesenchyme
TS13: embryo endoderm Present Regionally restricted 3F Expression in underlying ventral endoderm.
TS13: future hindbrain Present Regionally restricted 3F Expression in dorsal region of hindbrain.
Specimen 3G: embryonic day 8.5; Spry1tm1.1Jdli/Spry1+, Spry2tm1.1Mrt/Spry2+ (more )
Note: 12 somite stage.
Structure Level Pattern Image Note
TS13: otic placode Present Widespread 3G Expression in all cells of otic placode.
TS13: branchial arch endoderm Present 3G Expression in pharyngeal endoderm and adjacent pharyngeal mesenchyme
TS13: branchial arch mesenchyme Present 3G Expression in pharyngeal endoderm and adjacent pharyngeal mesenchyme
TS13: embryo endoderm Present Regionally restricted 3G Expression in underlying ventral endoderm.
TS13: future hindbrain Present Regionally restricted 3G Expression in dorsal region of hindbrain.
Specimen 3H: embryonic day 9.0; Spry1tm1.1Jdli/Spry1+, Spry2tm1.1Mrt/Spry2+ (more )
Note: 14 somite stage.
Structure Level Pattern Image Note
TS14: otic placode Present Regionally restricted 3H Expression enriched in medial regions of otic placode, closer to hindbrain.
TS14: otic placode mesenchyme Absent 3H No expression in underlying mesenchyme.
TS14: head surface ectoderm Ambiguous 3H No expresion in surface ectoderm immediately ventral to otic placode.
TS14: branchial arch endoderm Present 3H Expression in pharyngeal endoderm and adjacent pharyngeal mesenchyme
TS14: branchial arch mesenchyme Present 3H Expression in pharyngeal endoderm and adjacent pharyngeal mesenchyme
TS14: embryo endoderm Present Regionally restricted 3H Expression in underlying ventral endoderm.
TS14: future hindbrain Present Regionally restricted 3H Expression in dorsal region of hindbrain.
Specimen 3J: embryonic day 8.0; Spry1tm1.1Jdli/Spry1+, Spry2tm1.1Mrt/Spry2+ (more )
Note: 7 somite stage.
Structure Level Pattern Image Note
TS12: otic pre-placode Present 3J Broad domain of expression lateral to hindbrain.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory