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Gene Expression Data
Assay Details
Assay
Reference: J:187370 Keil KP, et al., Visualization and quantification of mouse prostate development by in situ hybridization. Differentiation. 2012 Oct;84(3):232-9
Assay type: RNA in situ
MGI Accession ID: MGI:6470423
Gene symbol: Nkx3-1
Gene name: NK3 homeobox 1
Probe: CVprobeNkx3-1(0143)
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: These authors used two probes against this gene in related publications; entries in the GUDMAP database identified this as the probe used to generate these data.
Results
Specimen 2A: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: epithelium of pelvic urethra of male Present Regionally restricted 2A Expression is seen in urogenital sinus epithelium. Expression is restricted to fields where ventral, anterior and dorsolateral prostate will arise.

Specimen 2D: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: anterior prostate bud Present 2D
TS24: dorsal prostate bud Present 2D Expression is concentrated where these buds will form.
TS24: lateral prostate bud Present 2D Expression is concentrated where these buds will form.
TS24: ventral prostate bud Present 2D Expression is concentrated where these buds will form.

Specimen 2G: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: anterior prostate bud Present 2G
TS25: dorsal prostate bud Present 2G
TS25: lateral prostate bud Present 2G
TS25: ventral prostate bud Present 2G

Specimen 2J: embryonic day 18.5 (more )
Note: This specimen was also stained with an antibody against Cdh1 (brown) to visualize the epithelium.
Structure Level Pattern Image Note
TS26: anterior prostate bud Present 2J
TS26: dorsal prostate bud Present 2J
TS26: lateral prostate bud Present 2J
TS26: ventral prostate bud Present 2J
TS26: male urethral gland bud Present 2J

Specimen 2J - inset: embryonic day 18.5 (more )
Note: This specimen was also stained with an antibody against Cdh1 (brown) to visualize the epithelium.
Structure Level Pattern Image Note
TS26: prostate bud Present Regionally restricted 2J inset Expression is detected in at least half of the length of the main prostatic buds and in all branched tips.

Specimen 2M: postnatal day 5 (more )
Note: This specimen was also stained with an antibody against Cdh1 (brown) to visualize the epithelium.
Structure Level Pattern Image Note
TS28: prostate gland Present 2M
TS28: male urethral gland Present 2M

Specimen 2M - inset: postnatal day 5 (more )
Note: This specimen was also stained with an antibody against Cdh1 (brown) to visualize the epithelium.
Structure Level Pattern Image Note
TS28: prostate epithelium Present Regionally restricted 2M inset Expression is detected in at least half of the length of the main prostatic buds and in all branched tips.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
01/28/2026
MGI 6.24
The Jackson Laboratory