Gad1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:287411 Tran HN, et al., Tcf7l2 transcription factor is required for the maintenance, but not the initial specification, of the neurotransmitter identity in the caudal thalamus. Dev Dyn. 2020 May;249(5):646-655
Assay type:	RNA in situ
MGI Accession ID:	MGI:6469621
Gene symbol:	Gad1
Gene name:	glutamate decarboxylase 1

Probe:	Gad1 probe6
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

1B ©

Specimen 1B: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Present		1B
TS20: forebrain-midbrain boundary region	Present	Regionally restricted	1B	Expressed in the pretectum.
TS20: thalamus	Present	Regionally restricted	1B	Expressed in the rostral thalamus.

1D ©

Specimen 1D: embryonic day 12.5; Tcf7l2^tm1Cle/Tcf7l2^tm1Cle (more

)

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Present		1D	Similar as in the wild-type.
TS20: forebrain-midbrain boundary region	Present	Regionally restricted	1D	Expressed in the pretectum, similar as in the wild-type.
TS20: thalamus	Present	Regionally restricted	1D	Expressed in the rostral thalamus, similar as in the wild-type.

1G ©

Specimen 1G: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	1G	Expressed in the rostral thalamus.
TS20: prethalamus	Present		1G

1H ©

Specimen 1H: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Present		1H	Similar as in the wild-type.
TS20: thalamus	Present	Regionally restricted	1H	Expressed in the rostral thalamus, similar as in the wild-type.

1K ©

Specimen 1K: embryonic day 12.5; Tcf7l2^tm1Cle/Tcf7l2^tm1Cle (more

)

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Present		1K
TS20: thalamus	Present	Regionally restricted	1K	Expressed in the rostral thalamus.
TS20: forebrain-midbrain boundary region	Present	Regionally restricted	1K	Expressed in the pretectum.

1L ©

Specimen 1L: embryonic day 12.5; Tcf7l2^tm1Cle/Tcf7l2^tm1Cle (more

)

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Present		1L	Similar as in the wild-type.
TS20: thalamus	Present	Regionally restricted	1L	Similar as in the wild-type. Expressed in the rostral thalamus.
TS20: forebrain-midbrain boundary region	Present	Regionally restricted	1L	Expressed in the pretectum, similar as in the wild-type.

3G ©

Specimen 3G: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: reticular thalamic nucleus	Present		3G

3H ©

Specimen 3H: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: reticular thalamic nucleus	Present		3H

3I ©

Specimen 3I: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: pretectum	Present		3I
TS24: ventral lateral geniculate nucleus	Present		3I
TS24: lateral geniculate nucleus	Present	Regionally restricted	3I	Expressed in the intergeniculate leaflet.

3M ©

Specimen 3M: embryonic day 16.5; Tcf7l2^tm1Cle/Tcf7l2^tm1Cle (more

)

Structure	Level	Pattern	Image	Note
TS24: reticular thalamic nucleus	Present		3M	Similar as in the wild-type.
TS24: thalamic nucleus	Present	Regionally restricted	3M	Ectopically detected in the marginal region of the caudal thalamus derivatives.

3N ©

Specimen 3N: embryonic day 16.5; Tcf7l2^tm1Cle/Tcf7l2^tm1Cle (more

)

Structure	Level	Pattern	Image	Note
TS24: reticular thalamic nucleus	Present		3N	Similar as in the wild-type.
TS24: thalamic nucleus	Present	Regionally restricted	3N	Ectopically detected in the marginal region of the caudal thalamus derivatives.

3O ©

Specimen 3O: embryonic day 16.5; Tcf7l2^tm1Cle/Tcf7l2^tm1Cle (more

)

Structure	Level	Pattern	Image	Note
TS24: pretectum	Present		3O	Similar as in the wild-type.
TS24: ventral lateral geniculate nucleus	Present		3O	Similar as in the wild-type.
TS24: thalamic nucleus	Present	Regionally restricted	3O	Ectopically detected in the marginal region of the caudal thalamus derivatives.
TS24: lateral geniculate nucleus	Present	Regionally restricted	3O	Expressed in the intergeniculate leaflet, similar as in the wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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