Isl2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:32806 Keller-Peck CR, et al., Patterns of neuronal differentiation in neural tube mutant mice: curly tail and Pax3 splotch-delayed. J Comp Neurol. 1996 May 13;368(4):516-26
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6467971
Gene symbol:	Isl2
Gene name:	insulin related protein 2 (islet 2)

Antibody:	clone 40.2D6
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Embryos were fixed with paraformaldehyde-lysine-periodate (PLP) fixative. They were embedded in polyester wax for sectioning.

Results

2A ©

Specimen 2A: embryonic day 12.5 (more

)
Note: phenotypically normal littermate control; transverse section

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventral horn	Present	Single cells	2A	Expression is seen in clusters of motorneurons.
TS20: spinal cord	Present	Single cells	2A	Expression is seen in scattered cells in the intermediate and dorsal regions.
TS20: dorsal root ganglion	Present	Single cells	2A

2B ©

Specimen 2B: embryonic day 12.5; Grhl3^ct/Grhl3^ct (more

)
Note: transverse section

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventral horn	Present	Single cells	2B	As in wild type, expression is seen in clusters of motorneurons.
TS20: spinal cord	Present	Single cells	2B	As in wild type, expression is seen in scattered cells in the intermediate and dorsal regions.
TS20: dorsal root ganglion	Present	Single cells	2B

2C ©

Specimen 2C: embryonic day 12.5 (more

)
Note: phenotypically normal littermate control; transverse section

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventral horn	Present	Single cells	2C	Expression is seen in clusters of motorneurons.
TS20: spinal cord	Present	Single cells	2C	Expression is seen in scattered cells in the intermediate and dorsal regions.
TS20: dorsal root ganglion	Present	Single cells	2C

2D ©

Specimen 2D: embryonic day 12.5; Pax3^Sp-d/Pax3^Sp-d (more

)
Note: transverse section

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventral horn	Present	Single cells	2D	As in wild type, expression is seen in clusters of motorneurons.
TS20: spinal cord	Present	Single cells	2D	As in wild type, expression is seen in scattered cells in the intermediate and dorsal regions.

Specimen not shown - ct control: embryonic day 13.5 (more

)
Note: phenotypically normal littermate control

Structure	Level	Pattern	Note
TS21: spinal cord intermediate grey	Present	Single cells	Expression is seen in the ventral and intermediate horn.
TS21: spinal cord ventral horn	Present	Single cells	Expression is seen in the ventral and intermediate horn.
TS21: dorsal root ganglion	Present	Single cells

Specimen not shown - ct: embryonic day 13.5; Grhl3^ct/Grhl3^ct (more

)

Structure	Level	Pattern	Note
TS21: spinal cord intermediate grey	Present	Single cells	As in wild type, expression is seen in the ventral and intermediate horn.
TS21: spinal cord ventral horn	Present	Single cells	As in wild type, expression is seen in the ventral and intermediate horn.
TS21: dorsal root ganglion	Present	Single cells

Specimen not shown - Sp-d control: embryonic day 13.5 (more

)
Note: phenotypically normal littermate control

Structure	Level	Pattern	Note
TS21: spinal cord intermediate grey	Present	Single cells	Expression is seen in the ventral and intermediate horn.
TS21: spinal cord ventral horn	Present	Single cells	Expression is seen in the ventral and intermediate horn.
TS21: dorsal root ganglion	Present	Single cells

Specimen not shown - Sp-d: embryonic day 13.5; Pax3^Sp-d/Pax3^Sp-d (more

)

Structure	Level	Pattern	Image	Note
TS21: spinal cord intermediate grey	Present	Single cells		As in wild type, expression is seen in the ventral and intermediate horn.
TS21: spinal cord ventral horn	Present	Single cells		As in wild type, expression is seen in the ventral and intermediate horn.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/18/2025 MGI 6.24