Antibody:	anti-aa2
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	1D , 2A	Expression is in a anteroposterior subregion positioned between the 4th and 10th prevertebrae and is weaker over cells of prevertebrae numbering 13-17.
TS20: vertebral pre-cartilage condensation	Present		1D , 2A

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	3A , 4A	Expression is in the ventral spinal cord with an anterior limit positioned at the level of the forelimb bud corresponding to the level of the 8th-12th somites.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	3B	Expression is as a stripe in the ventral spinal cord with an anterior limit positioned just posterior to the 6th prevertebra. Expression extends to the dorsal region in the most anterior domain of expression and continues as a stripe posteriorly.
TS19: vertebral pre-cartilage condensation	Present	Regionally restricted	3B	Expression is in the prevertebrae posterior to the most intense staining in the spinal cord (numbering 14-17).

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	3C , 4E	Expression extends to the level of the 5th vertebra in the ventral most spinal cord region, and to the level of the 3rd vertebra in the middle layer of the spinal cord. The most dorsal spinal cord has expression in a very narrow anteroposterior subregion between the 5th and 7th vertebrae. Posteriorly, strongest expression extends to the level of the 10th vertebra.

Structure	Level	Pattern	Image	Note
TS26: spinal cord	Present	Regionally restricted	4F , 3D	Expression is in the cervical cord region ranging roughly from the levels of the 2nd to the 8th vertebrae.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	4B	Expression is in the ventral spinal cord with an anterior limit positioned at the level of the forelimb bud corresponding to the level of the 8th-12th somites.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	4C	The posterior expression boundary overlaps with the mesodermal region of expression in prevertebrae numbered 14-17.
TS19: vertebral pre-cartilage condensation	Present	Regionally restricted	4C	Expression is in prevertebrae numbered 14-17.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	4D	Expression is in central and dorsally located spinal cord layers from the level of the 6th to the 4th prevertebra.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	5A' , 5A , 5A''	Expression is in two distinct ventro-lateral regions corresponding to the developing ventral horns and is weaker is in the more dorsal regions of the mantle layer and in undifferentiated ependymal cells.
TS17: spinal ganglion	Present		5A

Structure	Level	Pattern	Image	Note
TS19: neural tube basal columns	Present	Regionally restricted	5B'' , 5B , 5B'	Expression is in two separate subregions, located in the ventral most and middle areas on either side.
TS19: neural tube mantle layer	Present	Regionally restricted	5B'' , 5B , 5B'	Expression is in two separate subregions, located in the ventral most and middle areas on either side.
TS19: neural tube lateral wall	Present	Regionally restricted	5B' , 5B , 5B''	Expression is absent in almost all the cells of the ependymal region.
TS19: spinal ganglion	Weak		5B

Structure	Level	Pattern	Image	Note
TS22: spinal cord mantle layer	Present	Graded	5C , 5C'' , 5C'	Expression is ventral to dorsal and is absent in the central ependymal region.
TS22: ventral grey horn	Present		5C'' , 5C' , 5C

Structure	Level	Pattern	Image	Note
TS26: spinal cord mantle layer	Present	Graded	5D' , 5D'' , 5D	The highest concentration of expression is towards the ventral rergion and lower expression is in the dorsal mantle layer region and is absent in the central ependymal region.
TS26: ventral grey horn	Present		5D' , 5D , 5D''
TS26: dorsal grey horn	Present	Single cells	5D'' , 5D , 5D'

Structure	Level	Pattern	Image	Note
TS28: spinal cord dorsal horn	Present		6A'' , 6A , 6A'	Expression is nuclear and is absent in the nucleoli.
TS28: spinal cord ventral horn	Present		6A'' , 6A , 6A'	Expression is nuclear and is absent in the nucleoli.
TS28: sympathetic ganglion	Present	Single cells	6A'' , 6A , 6A'

Structure	Level	Pattern	Image	Note
TS28: spinal cord dorsal horn	Present		6B , 6B' , 6B''	Expression is nuclear and is absent in the nucleoli.
TS28: spinal cord ventral horn	Present		6B , 6B' , 6B''	Expression is nuclear and is absent in the nucleoli.