Gene Expression Data
Assay Details
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Assay
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Reference:
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J:162061
Slaten ER, et al., Transient expression of serotonin 5-HT4 receptors in the mouse developing thalamocortical projections. Dev Neurobiol. 2010 Feb 15;70(3):165-81
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6460413
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Gene symbol:
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Htr4
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Gene name:
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5 hydroxytryptamine (serotonin) receptor 4
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Antibody:
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SR-4 (C-18) antibody (sc-32566)
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Detection system:
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Secondary antibody coupled to other - see notes
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Assay notes:
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Secondary antibody labeled with quantum dot (QD) 655.
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Results
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Specimen
8E:
embryonic day 17.5
(more  )
Specimen
8E:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cerebral cortex intermediate zone |
Present |
Regionally restricted |
8E
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Expression in thalamocortical fibers detected with QD655 suggests receptors are not internalized since quantum dots are relatively large and cannot penetrate the cell membrane.
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| TS25: cerebral cortex marginal zone |
Absent |
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8E
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No expression in fibers in marginal zone.
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Analysis Tools
