Gene Expression Data
Assay Details
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Assay
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Reference:
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J:143569
Miller NL, et al., Necdin, a Prader-Willi syndrome candidate gene, regulates gonadotropin-releasing hormone neurons during development. Hum Mol Genet. 2009 Jan 15;18(2):248-60
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6458882
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Gene symbol:
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Gnrh1
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Gene name:
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gonadotropin releasing hormone 1
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Antibody:
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Anti-GnRH (PA1-121)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Fixative was: 10% acetic acid, 30% formaldehyde,
60% ethanol.
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Results
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Specimen
8B:
embryonic day 13.5
(more  )
Specimen
8B:
(close  )
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Genetic Background:
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involves: 129S1/Sv
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: head |
Present |
Regionally restricted |
8B
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Expression is in migrating neurons in the nasal and cribriform plate regions.
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| TS21: brain |
Present |
Regionally restricted |
8B
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Expression is in migrating neurons.
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Specimen
8C:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Ndntm2Stw/Ndntm2Stw
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: head |
Present |
Regionally restricted |
8C
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In this mutant the number of positive migrating neurons is significantly reduced in cribriform plate region (58%) and is similar to wild-type in the nasal region.
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| TS21: brain |
Present |
Regionally restricted |
8C
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The number of positive migrating neurons is significantly reduced (72%) in this mutant.
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Specimen
8G:
embryonic day 17.5
(more )
Specimen
8G:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: head |
Present |
Regionally restricted |
8G
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Expression is in migrating neurons in the nasal and cribriform plate regions.
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| TS25: brain |
Present |
Regionally restricted |
8G
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Expression is in migrating neurons.
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Specimen
8H:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Ndntm2Stw/Ndntm2Stw
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: head |
Present |
Regionally restricted |
8H
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The number of positive migrating neurons in cribriform plate region is similar to wild-type whereas expressing cells in the nasal region are increased in this mutant compared to wild-type.
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| TS25: brain |
Present |
Regionally restricted |
8H
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The number of positive neurons in the brain was 31% significantly lower in nthis mutant compared with wild-type embryos.
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Specimen
8K:
embryonic day 17.5
(more )
Specimen
8K:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
8L:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Ndntm2Stw/Ndntm2Stw
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Analysis Tools