About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:263032 Magella B, et al., Reduced Abd-B Hox function during kidney development results in lineage infidelity. Dev Biol. 2018 Jun 15;438(2):84-93
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6457096
Gene symbol: Krt8
Gene name: keratin 8
Antibody: TROMA 1
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 7A"/A'": embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: collecting duct Present 7A'" , 7A" Labeled for only a single segment type.
Specimen 7B"/B'": embryonic day 18.5; Hoxatm1.1Ssp/Hoxatm1.1Ssp, Hoxdtm1.1Ssp/Hoxdtm1.1Ssp (more )
Structure Level Pattern Image Note
TS26: collecting duct Present 7B" , 7B'" Most tubules included individual cells showing a mixed identity, simultaneously expressing markers of more than one tubule segment.
Specimen 7C"/C'": embryonic day 18.5; Hoxatm1.1Ssp/Hoxatm1.1Ssp, Hoxdtm1.1Ssp/Hoxdtm1.1Ssp (more )
Structure Level Pattern Image Note
TS26: collecting duct Present 7C" , 7C'" Most tubules included individual cells showing a mixed identity, simultaneously expressing markers of more than one tubule segment.
Specimen 9 E15.5 WT: embryonic day 15.5 (more )
Note: Triple labeled: blue - Krt8; green - LTA; red - Slc12a1 (assay MGI:6457107).
Structure Level Pattern Image Note
TS23: collecting duct Present 9_E15.5 left Labeled for only a single segment type.
Specimen 9 E15.5 mut: embryonic day 15.5; Hoxatm1.1Ssp/Hoxatm1.1Ssp, Hoxdtm1.1Ssp/Hoxdtm1.1Ssp (more )
Note: Triple labeled: blue - Krt8; green - LTA; red - Slc12a1 (assay MGI:6457107).
Structure Level Pattern Image Note
TS23: collecting duct Present 9_E15.5 right Mutant kidney tubules showed few examples of lineage infidelity.
Specimen 9 E16.5 WT: embryonic day 16.5 (more )
Note: Triple labeled: blue - Krt8; green - LTA; red - Slc12a1 (assay MGI:6457107).
Structure Level Pattern Image Note
TS24: collecting duct Present 9_E16.5 left Labeled for only a single segment type.
Specimen 9 E16.5 mut: embryonic day 16.5; Hoxatm1.1Ssp/Hoxatm1.1Ssp, Hoxdtm1.1Ssp/Hoxdtm1.1Ssp (more )
Note: Triple labeled: blue - Krt8; green - LTA; red - Slc12a1 (assay MGI:6457107).
Structure Level Pattern Image Note
TS24: collecting duct Present 9_E16.5 right There were some mutant tubules showing expression of markers of more than one tubule segment, but compared to E18.5 mutant kidneys there were relatively few.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory