About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:103362 Nakamura H, et al., Developmentally regulated expression of Sp1 in the mouse cornea. Invest Ophthalmol Vis Sci. 2005 Nov;46(11):4092-6
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6456780
Gene symbol: Sp1
Gene name: trans-acting transcription factor 1
Antibody: anti-Sp1
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Results
Specimen 1/Table 1 - E10.5: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: lens pit Moderate Regionally restricted 1_E10.5 Expression observed in the future lens epithelium.

Specimen 1/Table 1 - E12.5: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: cornea epithelium Strong 1_E12.5 Nuclear expression observed in the ectoderm.
TS20: cornea stroma Strong 1_E12.5 Nuclear expression observed in the mesenchyme (future corneal stroma and endothelium).
TS20: lens vesicle epithelium Moderate 1_E12.5

Specimen 1/Table 1 - E15.5: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: cornea epithelium Strong 1_E15.5 Nuclear staining observed in the basal corneal epithelium.
TS23: cornea stroma Strong 1_E15.5 Nuclear staining observed.
TS23: lens epithelium Weak 1_E15.5

Specimen 1/Table 1 - E18.5: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: cornea epithelium Strong 1_E18.5 Nuclear staining is observed in the basal corneal epithelium.
TS26: cornea stroma Moderate 1_E18.5 Nuclear staining is observed.
TS26: lens epithelium Trace 1_E18.5

Specimen 1/Table 1 - P0: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: cornea epithelium Strong 1_P0 Nuclear staining observed in the basal corneal epithelium.
TS27: cornea stroma Moderate 1_P0 Nuclear staining observed.
TS27: cornea endothelium Trace 1_P0
TS27: lens epithelium Trace 1_P0 Staining very faint or absent.

Specimen 1/Table 1 - P7: postnatal day 7 (more )
Structure Level Pattern Image Note
TS28: cornea epithelium Strong 1_P7 Nuclear staining observed in the basal corneal epithelium.
TS28: cornea stroma Strong 1_P7 Nuclear staining observed.
TS28: cornea endothelium Trace 1_P7
TS28: lens epithelium Trace 1_P7 Staining very faint or absent.

Specimen 1/Table 1 - P11: postnatal day 11 (more )
Structure Level Pattern Image Note
TS28: cornea epithelium Strong 1_P11 Nuclear staining observed seen in the basal corneal epithelium.
TS28: cornea stroma Strong 1_P11 Nuclear staining observed.
TS28: cornea endothelium Moderate 1_P11 Staining weak or moderate.
TS28: lens epithelium Trace 1_P11 Staining very faint or absent.

Specimen 1/Table 1 - P15: postnatal day 15 (more )
Note: eyelid opening occurs at P13-14
Structure Level Pattern Image Note
TS28: cornea epithelium Weak 1_P15 At this age expression in the basal corneal epithelial cells and karatocytes is much reduced.
TS28: cornea stroma Weak 1_P15
TS28: cornea endothelium Trace 1_P15
TS28: lens epithelium Trace 1_P15 Staining very faint or absent.

Specimen 1/Table 1 - P30: postnatal day 30 (more )
Structure Level Pattern Image Note
TS28: cornea epithelium Trace Single cells 1_P30 Strong staining is seen in a few superficial cells.
TS28: cornea stroma Weak 1_P30
TS28: cornea endothelium Trace 1_P30
TS28: lens epithelium Trace 1_P30 Staining very faint or absent.

Specimen 1/Table 1 - P60: postnatal day 60 (more )
Structure Level Pattern Image Note
TS28: cornea epithelium Trace Single cells 1_P60 Strong staining is seen in a few superficial cells.
TS28: cornea stroma Weak 1_P60
TS28: cornea endothelium Trace 1_P60
TS28: lens epithelium Trace 1_P60 Staining very faint or absent.

Specimen 3 - P0: postnatal day 0 (more )
Note: mouse bred in the dark
Structure Level Pattern Image Note
TS27: cornea epithelium Strong 3_P0 Moderate to strong expression seen in basal corneal epithelial cells and keratocytes; the expression pattern is nearly identical to mice bred in a light/dark cycle.

Specimen 3 - P7: postnatal day 7 (more )
Note: mouse bred in the dark
Structure Level Pattern Image Note
TS28: cornea epithelium Strong 3_P7 Moderate to strong expression seen in basal corneal epithelial cells and keratocytes; the expression pattern is nearly identical to mice bred in a light/dark cycle.

Specimen 3 - P11: postnatal day 11 (more )
Note: mouse bred in the dark
Structure Level Pattern Image Note
TS28: cornea epithelium Strong 3_P11 Moderate to strong expression seen in basal corneal epithelial cells and keratocytes; the expression pattern is nearly identical to mice bred in a light/dark cycle.

Specimen 3 - P15: postnatal day 15 (more )
Note: mouse bred in the dark
Structure Level Pattern Image Note
TS28: cornea epithelium Weak 3_P15 The expression pattern is nearly identical to mice bred in a light/dark cycle.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/25/2025
MGI 6.24
The Jackson Laboratory