Probe:	Nhlh1 probe2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent		2C

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present
TS15: trigeminal preganglion	Present
TS15: central nervous system	Present	Regionally restricted		Expression in rostral 2/3rds of neural tube.

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present		2D
TS15: trigeminal preganglion	Present		2D
TS15: central nervous system	Present	Regionally restricted	2D	Expression in rostral 2/3rds of neural tube.

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present		2E
TS15: trigeminal preganglion	Present		2E
TS15: central nervous system	Present	Regionally restricted	2E	Expression in rostral 2/3rds of neural tube.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted		At level of heart, expression at lateral edge of ventricular zone in ventral half of neural tube.
TS15: neural tube floor plate	Absent

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	2F	At level of heart, expression at lateral edge of ventricular zone in ventral half of neural tube.
TS15: neural tube floor plate	Absent		2F

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	2G	At level of heart, expression at lateral edge of ventricular zone in ventral half of neural tube.
TS15: neural tube floor plate	Absent		2G

Structure	Level	Pattern	Image	Note
TS17: facio-acoustic ganglion complex	Strong		3A	Increased intensity of expression in trigeminal and facio-acoustic ganglia.
TS17: trigeminal ganglion	Strong		3A	Increased intensity of expression in trigeminal and facio-acoustic ganglia.
TS17: neural tube	Strong		3A	Increased intensity of expression in neural tube.

Structure	Level	Pattern	Image	Note
TS17: facio-acoustic ganglion complex	Strong		3B	Increased intensity of expression in trigeminal and facio-acoustic ganglia.
TS17: trigeminal ganglion	Strong		3B	Increased intensity of expression in trigeminal and facio-acoustic ganglia.
TS17: neural tube	Strong		3B	Increased intensity of expression in neural tube.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted		At level of the heart, expression in ventral half of neural tube at lateral edge of ventricular zone. Expression also in dorsal 1/5th of neural tube, at lateral edge of ventricular zone. Region between dorsal and ventral domains absent for expression.
TS17: neural tube floor plate	Absent
TS17: neural tube roof plate	Absent

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3C	At level of the heart, expression in ventral half of neural tube at lateral edge of ventricular zone. Expression also in dorsal 1/5th of neural tube, at lateral edge of ventricular zone. Region between dorsal and ventral domains absent for expression.
TS17: neural tube floor plate	Absent		3C
TS17: neural tube roof plate	Absent		3C

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3D	At level of the heart, expression in ventral half of neural tube at lateral edge of ventricular zone. Expression also in region corresponding to dorsal neural tube, at lateral edge of ventricular zone. Region between dorsal and ventral domains absent for expression.
TS17: neural tube floor plate	Absent		3D
TS17: neural tube roof plate	Absent		3D

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3E	At more caudal level, expression only in ventral half of neural tube, at lateral edge of ventricular zone. Expression in few cells more medially, within area of ventricular zone.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3F	At even more caudal level, Expression is not detectable.

Structure	Level	Pattern	Image	Note
TS17: facio-acoustic ganglion complex	Present		4A
TS17: trigeminal ganglion	Present		4A
TS17: central nervous system	Present		4A
TS17: peripheral nervous system	Present	Regionally restricted	4A	Expression in streams of cells entering the heart, corresponding to the vagus nerve.
TS17: dorsal root ganglion	Present	Regionally restricted	4A	Higher levels of expression in more rostral dorsal root ganglia (DRG); lower levels in more caudal DRG.

Structure	Level	Pattern	Image	Note
TS17: facio-acoustic ganglion complex	Present		4B
TS17: trigeminal ganglion	Present		4B
TS17: central nervous system	Present		4B
TS17: peripheral nervous system	Present	Regionally restricted	4B	Expression in streams of cells entering the heart, corresponding to the vagus nerve.
TS17: dorsal root ganglion	Present	Regionally restricted	4B	Higher levels of expression in more rostral dorsal root ganglia (DRG); lower levels in more caudal DRG.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	4C	At level of forelimb bud, stripe of expression along lateral edge of ventricular zone, interrupted by a region lacking expression immediately dorsal to presumptive sulcus limitans. No expression in ventrolateral area of neural tube.
TS17: neural tube floor plate	Absent		4C
TS17: neural tube roof plate	Absent		4C
TS17: dorsal root ganglion	Present		4C

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	4D	At level of forelimb bud, stripe of expression along lateral edge of ventricular zone, interrupted by a region lacking expression immediately dorsal to presumptive sulcus limitans. No expression in ventrolateral area of neural tube.
TS17: neural tube floor plate	Absent		4D
TS17: neural tube roof plate	Absent		4D
TS17: dorsal root ganglion	Present		4D

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	4E	At level of hindlimb bud, expression restricted to ventral 1/4 of neural tube. Expression detected more medially, not just restricted to lateral edge.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	4F	Expression at lateral edge of ventricular zone. No expression in ventrolateral region of neural tube.
TS17: dorsal root ganglion	Present		4F

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	4G	Expression at lateral edge of ventricular zone. No expression in ventrolateral region of neural tube.
TS17: dorsal root ganglion	Present		4G