Nefm Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:160737 Heupel K, et al., Loss of transforming growth factor-beta 2 leads to impairment of central synapse function. Neural Dev. 2008;3:25
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6450893
Gene symbol:	Nefm
Gene name:	neurofilament, medium polypeptide

Antibody:	anti-NF150
Detection system:	Secondary antibody coupled to Cy3

Results

2a WT ©

Specimen 2a WT: embryonic day 18.5 (more

)

Structure	Level	Pattern	Image	Note
TS26: phrenic nerve	Present		2a WT

2a KO ©

Specimen 2a KO: embryonic day 18.5; Tgfb2^tm1Doe/Tgfb2^tm1Doe (more

)

Structure	Level	Pattern	Image	Note
TS26: phrenic nerve	Present		2a KO	Branching of phrenic nerve into 3 primary branches as in wild-type.

2b WT ©

Specimen 2b WT: embryonic day 18.5 (more

)
Note: Double labeled: red - Nefm; green - alpha bungarotoxin.

Structure	Level	Pattern	Image	Note
TS26: phrenic nerve	Present		2b WT

2b KO ©

Specimen 2b KO: embryonic day 18.5; Tgfb2^tm1Doe/Tgfb2^tm1Doe (more

)
Note: Double labeled: red - Nefm; green - alpha bungarotoxin.

Structure	Level	Pattern	Image	Note
TS26: phrenic nerve	Present		2b KO	Fasciculation of phrenic nerve as in wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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