Antibody:	anti-villin R200.2
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present	Diffuse	2A/B	Diffuse cytoplasmic expression in primitive endoderm in cells lining blastocoelic surface of inner cell mass. No expression in primitive endoderm cells loosely associated with lateral sides of inner cell mass.

Structure	Level	Pattern	Image	Note
TS8: primitive endoderm	Present	Regionally restricted	2C	Expression in visceral endoderm surrounding primitive ectoderm.
TS8: parietal endoderm	Absent		2C

Structure	Level	Pattern	Image	Note
TS9: primitive endoderm	Present	Regionally restricted	2D/E	Expression in visceral endoderm in contact with both embryonic and extraembryonic ectoderm. Expression concentrated in apical cytoplasm.
TS9: embryo ectoderm	Absent		2D/E
TS9: extraembryonic ectoderm	Absent		2D/E
TS9: parietal endoderm	Absent		2D/E

Structure	Level	Pattern	Image	Note
TS13: yolk sac endoderm	Present		3B (left)	Expression in visceral endoderm as it differentiated into epithelial lining of visceral yolk sac.
TS13: foregut diverticulum	Absent		3B (left)	No expression in invaginating foregut.

Structure	Level	Pattern	Image	Note
TS13: yolk sac endoderm	Present		3B (right)	Expression prominently localized in apical cytoplasm layer of visceral yolk sac.
TS13: hindgut diverticulum	Absent		3B (right)	No expression in hindgut.

Structure	Level	Pattern	Image	Note
TS17: duodenum	Present	Regionally restricted	7A	Expression first apparent in intestinal epithelium. Mottled expression pattern in intestinal cells, localized to cell boundaries.
TS17: gut mesenchyme	Absent		7A	No expression in underlying mesenchyme or connective tissues.

Structure	Level	Pattern	Image	Note
TS20: duodenum epithelium	Present	Regionally restricted	7B	Expression concentrated in apical cytoplasm along luminal surface of intestine.

Structure	Level	Pattern	Image	Note
TS23: duodenum epithelium	Present	Regionally restricted	7C	Expression concentrated in apical cytoplasm.

Structure	Level	Pattern	Image	Note
TS28: duodenum epithelium	Present	Regionally restricted	7D	Expression localized in apical cytoplasm in the epithelial lining of the adult gut.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Absent
TS28: ovary	Absent