Probe:	probe 2
Probe preparation:	Antisense labelled with radioactivity RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present		1A/B	Higher expression.
TS13: embryo mesoderm	Present		1A/B	Higher expression in the mesoderm of the posterior regions.
TS13: heart	Absent		1A/B
TS13: unsegmented mesenchyme	Weak		1A/B

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		1D/E	Expression was higher in the posterior region.
TS13: future brain neural fold	Present		1D/E	Expression was weak in the posterior region.
TS13: yolk sac endoderm	Present	Regionally restricted	1D/E	Expressed in the visceral yolk sac and parietal yolk sac.
TS13: future hindbrain	Weak		1D/E
TS13: unsegmented mesenchyme	Weak		1D/E

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	4A/B	Expressed predominantly in the dorsolateral regions.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	4C/D	Expressed predominantly in the dorsolateral regions.

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Strong	Regionally restricted	5A	Expressed in the posterior (caudal) hindbrain.
TS21: spinal cord	Strong	Regionally restricted	5A	Strong expression only to the level of the second cervical somite. Posterior to this point, expression was weaker.
TS21: medulla oblongata	Strong		5A	No change in the anterior limit of high expression was observed.
TS21: lung mesenchyme	Present		5A
TS21: metanephros	Present		5A
TS21: gut	Present	Punctate	5A	Expressed within the muscle layers, corresponding to ganglia of the myenteric (Auerbach's) plexus.
TS21: heart	Absent		5A
TS21: liver	Absent		5A

Structure	Level	Pattern	Image	Note
TS23: hindbrain	Present	Regionally restricted	5C	Expressed in the posterior.
TS23: spinal cord	Present	Regionally restricted	5C	Expressed in the anterior.
TS23: gut	Present	Punctate	5C	Expressed around the loops of the gut. Expressed within the muscle layers, corresponding to ganglia of the myenteric (Auerbach's) plexus.
TS23: lung	Weak		5C
TS23: metanephros	Weak		5C
TS23: heart	Absent		5C
TS23: liver	Absent		5C

Structure	Level	Pattern	Image	Note
TS20: lung mesenchyme	Strong	Regionally restricted	7A/B	Expressed in the mesodermal layer, with strongest expression around the tips of the growing buds.

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent

Structure	Level	Pattern	Image	Note
TS10: embryo	Absent

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present	Regionally restricted		Expressed in the posterior.
TS22: spinal cord	Present	Regionally restricted		Expressed in the anterior.

Structure	Level	Pattern	Image	Note
TS27: hindbrain	Weak	Regionally restricted		Expressed in the posterior.
TS27: spinal cord	Weak	Regionally restricted		Expressed in the anterior.