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Gene Expression Data
Assay Details
Assay
Reference: J:277394 Tellier AP, et al., The elongation factor Elof1 is required for mammalian gastrulation. PLoS One. 2019;14(7):e0219410
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6446220
Gene symbol: Elof1
Gene name: ELF1 homolog, elongation factor 1
Reporter: lacZ
Direct Detection
Assay notes: Embryos were fixed in a buffer containing 0.2% glutaraldehyde and 1% formaldehyde.
Results
Specimen 2A: embryonic day 6.5; Elof1tm1.1(KOMP)Vlcg/Elof1+ (more )
Structure Level Pattern Image Note
TS9: embryo Present 2A
Specimen 2A': embryonic day 6.5; Elof1tm1.1(KOMP)Vlcg/Elof1+ (more )
Note: sagittal section
Structure Level Pattern Image Note
TS9: embryo Present 2A' Expression is strongest in the epiblast.
TS9: primitive endoderm Absent 2A' There is a lack of expression in the visceral endoderm. Any weak puncta of X-gal are thought to be remnants of expression at earlier stages.
Specimen 2B: embryonic day 7.5; Elof1tm1.1(KOMP)Vlcg/Elof1+ (more )
Structure Level Pattern Image Note
TS11: embryo Present 2B Expression is seen in all epiblast derivatives.
Specimen 2B': embryonic day 7.5; Elof1tm1.1(KOMP)Vlcg/Elof1+ (more )
Note: sagittal section
Structure Level Pattern Image Note
TS11: definitive endoderm Strong 2B'
TS11: embryo mesoderm Strong 2B'
TS11: extraembryonic mesoderm Strong 2B'
TS11: neural ectoderm Strong 2B'
TS11: node Strong 2B'
TS11: primitive streak Strong 2B'
Specimen 2C: embryonic day 8.5; Elof1tm1.1(KOMP)Vlcg/Elof1+ (more )
Structure Level Pattern Image Note
TS13: embryo Present 2C Fewer tissues show expression at E8.5 than at E7.5.
Specimen 2C': embryonic day 8.5; Elof1tm1.1(KOMP)Vlcg/Elof1+ (more )
Note: sagittal section
Structure Level Pattern Image Note
TS13: embryo Present 2C' The majority of tissues that showed expression at E7.5 now show no or weak expression.
Specimen 2C'': embryonic day 8.5; Elof1tm1.1(KOMP)Vlcg/Elof1+ (more )
Note: transverse section
Structure Level Pattern Image Note
TS13: notochord Strong 2C''
TS13: neural tube floor plate Strong Regionally restricted 2C'' Expression is seen in the floor plate of the posterior neural tube.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory