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Gene Expression Data
Assay Details
Assay
Reference: J:290235 Archambault D, et al., Protein phosphatase 1 regulatory subunit 35 is required for ciliogenesis, notochord morphogenesis, and cell-cycle progression during murine development. Dev Biol. 2020 Jul 3;465(1):1-10
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6446121
Gene symbol: T
Gene name: brachyury, T-box transcription factor T
Antibody: anti-Brachury (N-19; sc-17743)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 4K: embryonic day 7.5 (more )
Note: Littermate control; this embryo might have been a heterozygote. Sagittal section.
Structure Level Pattern Image Note
TS11: primitive streak Present 4K
TS11: embryo mesoderm Present 4K
Specimen 4L: embryonic day 7.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35tm1.1(KOMP)Vlcg (more )
Note: Sagittal section.
Structure Level Pattern Image Note
TS11: primitive streak Present 4L At this age expression in the mutant is similar to wild type.
TS11: embryo mesoderm Present 4L At this age expression in the mutant is similar to wild type.
Specimen 4M: embryonic day 8.5 (more )
Note: Littermate control; this embryo might have been a heterozygote. Transverse section.
Structure Level Pattern Image Note
TS13: notochord Present 4M Expression is restricted to the notochord at this age.
Specimen 4N: embryonic day 9.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35tm1.1(KOMP)Vlcg (more )
Note: Transverse section.
Structure Level Pattern Image Note
TS13: notochord Present Non-Uniform 4N The notochord is fragmented in the mutant. In addition, a developmental delay is observed at this age.
Specimen 4N': embryonic day 9.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35tm1.1(KOMP)Vlcg (more )
Note: Transverse section.
Structure Level Pattern Image Note
TS13: notochord Absent 4N' The notochord is fragmented in the mutants. In addition, a developmental delay is observed at this age.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
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