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Gene Expression Data
Assay Details
Assay
Reference: J:290235 Archambault D, et al., Protein phosphatase 1 regulatory subunit 35 is required for ciliogenesis, notochord morphogenesis, and cell-cycle progression during murine development. Dev Biol. 2020 Jul 3;465(1):1-10
Assay type: RNA in situ
MGI Accession ID: MGI:6446120
Gene symbol: Shh
Gene name: sonic hedgehog
Probe: Shh cDNA2
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 4A: embryonic day 7.5 (more )
Note: Littermate control; this embryo might have been a heterozygote.
Structure Level Pattern Image Note
TS11: notochordal plate Present 4A
Specimen 4B: embryonic day 7.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35tm1.1(KOMP)Vlcg (more )
Structure Level Pattern Image Note
TS11: embryo Absent 4B
Specimen 4C: embryonic day 8.0 (more )
Note: Littermate control; this embryo might have been a heterozygote.
Structure Level Pattern Image Note
TS12: notochordal plate Present 4C
Specimen 4D: embryonic day 8.0; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35tm1.1(KOMP)Vlcg (more )
Structure Level Pattern Image Note
TS11: notochordal plate Present 4D A developmental delay is observed in the mutants.
Specimen 4E: embryonic day 8.5 (more )
Note: Littermate control; this embryo might have been a heterozygote.
Structure Level Pattern Image Note
TS13: notochord Present 4E
Specimen 4F: embryonic day 8.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35tm1.1(KOMP)Vlcg (more )
Structure Level Pattern Image Note
TS12: notochordal plate Present 4F A developmental delay is observed in the mutants.
Specimen 4G: embryonic day 9.5 (more )
Note: Littermate control; this embryo might have been a heterozygote.
Structure Level Pattern Image Note
TS15: floor plate Present 4G
TS15: notochord Present 4G
TS15: embryo endoderm Present 4G
Specimen 4H: embryonic day 9.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35tm1.1(KOMP)Vlcg (more )
Structure Level Pattern Image Note
TS13: notochord Present Non-Uniform 4H Unlike in the controls, expression is discontinuous in the presumptive notochord.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
MGI 6.24 		The Jackson Laboratory