Gene Expression Data
Assay Details
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Assay
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Reference:
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J:231312
Baardman ME, et al., Common arterial trunk and ventricular non-compaction in Lrp2 knockout mice indicate a crucial role of LRP2 in cardiac development. Dis Model Mech. 2016 Apr 1;9(4):413-25
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6444474
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Gene symbol:
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Nkx2-5
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Gene name:
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NK2 homeobox 5
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Results
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Specimen
4E:
embryonic day 10.5
(more  )
Specimen
4E:
(close  )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: secondary heart field |
Present |
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4E
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A population of second heart field cells was seen to cross from dorsal to ventral, along the 4th pharyngeal arch artery on the right (aortic) side of the aortic sac up to the myocardial borderline of the aortic sac. Another second heart field cell population in front of the gut formed the flow divider and was negative for Tfap2a.
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Specimen
4G:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Lrp2tm1Her/Lrp2tm1Her
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: secondary heart field |
Present |
Regionally restricted |
4G
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The anteriorly located population directed towards the right (aortic side) of the outflow tract was less well developed and less organized in the mutant embryo compared to the wild type.
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Specimen
4I:
embryonic day 10.5
(more )
Specimen
4I:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: secondary heart field |
Present |
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4I
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On the left (pulmonary) side of the aortic sac below the 6th pharyngeal arch artery, a second heart field population was seen.
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Specimen
4K:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Lrp2tm1Her/Lrp2tm1Her
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: secondary heart field |
Present |
Regionally restricted |
4K
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The development of the left-sided population that leads to the pulmonary push was diminished.
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Specimen
5E:
embryonic day 11.5
(more )
Specimen
5E:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: cardiac muscle tissue |
Present |
Regionally restricted |
5E
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Expressed in a stream of second heart field cells coursing between the 4th and 6th pharyngeal arch arteries.
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Specimen
5F:
embryonic day 11.5
(more )
Specimen
5F:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: cardiac muscle tissue |
Present |
Regionally restricted |
5F
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Expressed in a stream of second heart field cells coursing between the 4th and 6th pharyngeal arch arteries, and the flow divider that moved to the left.
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Specimen
5H:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Lrp2tm1Her/Lrp2tm1Her
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: cardiac muscle tissue |
Present |
Regionally restricted |
5H
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The dorsal population remained in midline, resulting in an absence of the extension along the left (pulmonary) side.
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Specimen
5I:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Lrp2tm1Her/Lrp2tm1Her
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: cardiac muscle tissue |
Present |
Regionally restricted |
5I
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The dorsal population remained in midline, resulting in an absence of the extension along the left (pulmonary) side.
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