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Gene Expression Data
Assay Details
Assay
Reference: J:290235 Archambault D, et al., Protein phosphatase 1 regulatory subunit 35 is required for ciliogenesis, notochord morphogenesis, and cell-cycle progression during murine development. Dev Biol. 2020 Jul 3;465(1):1-10
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6444130
Gene symbol: Ppp1r35
Gene name: protein phosphatase 1, regulatory subunit 35
Reporter: lacZ
Direct Detection
Assay notes: Embryos were fixed in a buffer containing 0.2% glutaraldehyde and 1% formaldehyde.
Results
Specimen 1A: embryonic day 6.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35+ (more )
Structure Level Pattern Image Note
TS8: embryo Present 1A
TS8: extraembryonic component Present Regionally restricted 1A Expression is seen in some cells.
Specimen 1B: embryonic day 7.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35+ (more )
Structure Level Pattern Image Note
TS11: embryo Present Widespread 1B Expression is seen in all nascent embryonic derivatives.
Specimen 1C: embryonic day 8.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35+ (more )
Structure Level Pattern Image Note
TS13: embryo Present 1C
Specimen 1D: embryonic day 9.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35+ (more )
Structure Level Pattern Image Note
TS15: embryo Present Widespread 1D
Specimen 1E: embryonic day 6.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35+ (more )
Note: sagittal section
Structure Level Pattern Image Note
TS8: embryo Present 1E
TS8: extraembryonic ectoderm Present Regionally restricted 1E Expression is seen in some cells.
TS8: primitive endoderm Absent 1E There is no expression in the visceral endoderm.
Specimen 1F: embryonic day 7.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35+ (more )
Note: sagittal section
Structure Level Pattern Image Note
TS11: embryo Present 1F
TS11: primitive streak Strong 1F
TS11: allantois Strong 1F
TS11: extraembryonic visceral endoderm Absent 1F
Specimen 1G: embryonic day 8.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35+ (more )
Note: sagittal section
Structure Level Pattern Image Note
TS13: heart Present 1G
TS13: somite Present 1G
Specimen 1H: embryonic day 9.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35+ (more )
Note: sagittal section
Structure Level Pattern Image Note
TS15: heart Present 1H
TS15: somite Present 1H
Specimen 1I: embryonic day 6.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35+ (more )
Note: transverse section
Structure Level Pattern Image Note
TS8: epiblast Present 1I
Specimen 1J: embryonic day 7.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35+ (more )
Note: transverse section
Structure Level Pattern Image Note
TS11: allantois Strong 1J
TS11: embryo ectoderm Strong 1J
TS11: embryo mesoderm Strong 1J
Specimen 1K: embryonic day 8.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35+ (more )
Note: transverse section
Structure Level Pattern Image Note
TS13: heart Present 1K
TS13: neural tube Present 1K
TS13: notochord Present 1K
TS13: gut Ambiguous 1K Expression is relatively absent at this age.
Specimen 1L: embryonic day 9.5; Ppp1r35tm1.1(KOMP)Vlcg/Ppp1r35+ (more )
Note: transverse section
Structure Level Pattern Image Note
TS15: heart Present 1L
TS15: neural tube Present 1L
TS15: notochord Present 1L
TS15: foregut Present 1L
TS15: hindgut Absent 1L
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
MGI 6.13 		The Jackson Laboratory