Gene Expression Data
Assay Details
Assay
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Reference:
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J:265803
Da Silva-Alverez S, et al., Pkd2 deletion during embryo development does not alter mesonephric programmed cell senescence. Int J Dev Biol. 2018;62(9-10):637-640
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6441157
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Gene symbol:
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Mki67
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Gene name:
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antigen identified by monoclonal antibody Ki 67
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Antibody:
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anti-Ki-67 (MAD-0003110QD)
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Detection system:
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horseradish peroxidase
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Assay notes:
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Fixation with 2% formaldehyde/0.2% glutaraldehyde.
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Results
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Specimen
2A WT E13.5 Ki67:
embryonic day 13.5; Pkd2tm2Som/Pkd2 +
(more )
Note: Double labeled: brown - Mki67; blue - Pkd2 reporter (assay MGI:6441151).
Specimen
2A WT E13.5 Ki67:
(close )
Genetic Background:
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involves: 129/Sv * C57BL/6J * SJL
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Mutant Allele(s):
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Pkd2tm2Som/Pkd2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Double labeled: brown - Mki67; blue - Pkd2 reporter (assay MGI:6441151).
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Specimen
2A Pkd2-null E13.5 Ki67:
embryonic day 13.5; Pkd2tm1Som/ Pkd2tm2Som
(more )
Note: Double labeled: brown - Mki67; blue - Pkd2 reporter (assay MGI:6441151).
Specimen
2A Pkd2-null E13.5 Ki67:
(close )
Genetic Background:
|
involves: 129/Sv * C57BL/6J * SJL
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Mutant Allele(s):
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Pkd2tm1Som/Pkd2tm2Som
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Double labeled: brown - Mki67; blue - Pkd2 reporter (assay MGI:6441151).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: endolymphatic sac |
Absent |
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2A Pkd2-null E13.5 Ki67
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Exact same pattern as in wild type embryos.
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Specimen
2A WT E14.5 Ki67:
embryonic day 14.5; Pkd2tm2Som/Pkd2 +
(more )
Note: Double labeled: brown - Mki67; blue - Pkd2 reporter (assay MGI:6441151).
Specimen
2A WT E14.5 Ki67:
(close )
Genetic Background:
|
involves: 129/Sv * C57BL/6J * SJL
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Mutant Allele(s):
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Pkd2tm2Som/Pkd2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Double labeled: brown - Mki67; blue - Pkd2 reporter (assay MGI:6441151).
|
Specimen
2A Pkd2-null E14.5 Ki67:
embryonic day 14.5; Pkd2tm1Som/ Pkd2tm2Som
(more )
Note: Double labeled: brown - Mki67; blue - Pkd2 reporter (assay MGI:6441151).
Specimen
2A Pkd2-null E14.5 Ki67:
(close )
Genetic Background:
|
involves: 129/Sv * C57BL/6J * SJL
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Mutant Allele(s):
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Pkd2tm1Som/Pkd2tm2Som
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Double labeled: brown - Mki67; blue - Pkd2 reporter (assay MGI:6441151).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: endolymphatic sac |
Absent |
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2A Pkd2-null E14.5 Ki67
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Exact same pattern as in wild type embryos.
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Specimen
2B WT E13.5 Ki67:
embryonic day 13.5; Pkd2tm2Som/Pkd2 +
(more )
Note: Double labeled: brown - Mki67; blue - Pkd2 reporter (assay MGI:6441151).
Specimen
2B WT E13.5 Ki67:
(close )
Genetic Background:
|
involves: 129/Sv * C57BL/6J * SJL
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Mutant Allele(s):
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Pkd2tm2Som/Pkd2+
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Age:
|
embryonic day 13.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: brown - Mki67; blue - Pkd2 reporter (assay MGI:6441151).
|
Specimen
2B Pkd2-null E13.5 Ki67:
embryonic day 13.5; Pkd2tm1Som/ Pkd2tm2Som
(more )
Note: Double labeled: brown - Mki67; blue - Pkd2 reporter (assay MGI:6441151).
Specimen
2B Pkd2-null E13.5 Ki67:
(close )
Genetic Background:
|
involves: 129/Sv * C57BL/6J * SJL
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Mutant Allele(s):
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Pkd2tm1Som/Pkd2tm2Som
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Age:
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embryonic day 13.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
|
Double labeled: brown - Mki67; blue - Pkd2 reporter (assay MGI:6441151).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: mesonephric tubule |
Absent |
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2B Pkd2-null E13.5 Ki67
|
Exact same pattern as in wild type embryos.
|
Specimen
2B WT E14.5 Ki67:
embryonic day 14.5; Pkd2tm2Som/Pkd2 +
(more )
Note: Double labeled: brown - Mki67; blue - Pkd2 reporter (assay MGI:6441151).
Specimen
2B WT E14.5 Ki67:
(close )
Genetic Background:
|
involves: 129/Sv * C57BL/6J * SJL
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Mutant Allele(s):
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Pkd2tm2Som/Pkd2+
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Age:
|
embryonic day 14.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: brown - Mki67; blue - Pkd2 reporter (assay MGI:6441151).
|
Specimen
2B Pkd2-null E14.5 Ki67:
embryonic day 14.5; Pkd2tm1Som/ Pkd2tm2Som
(more )
Note: Double labeled: brown - Mki67; blue - Pkd2 reporter (assay MGI:6441151).
Specimen
2B Pkd2-null E14.5 Ki67:
(close )
Genetic Background:
|
involves: 129/Sv * C57BL/6J * SJL
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Mutant Allele(s):
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Pkd2tm1Som/Pkd2tm2Som
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
|
Note:
|
Double labeled: brown - Mki67; blue - Pkd2 reporter (assay MGI:6441151).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: mesonephric tubule |
Absent |
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2B Pkd2-null E14.5 Ki67
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Exact same pattern as in wild type embryos.
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