Antibody:	anti-Tbr1 (AB31940)
Detection system:	Secondary antibody coupled to Alexa Fluor 555
Assay notes:	Another Ptprd knockout allele (Ptprd<tm2a(KOMP)Wtsi>) was mentioned in the paper. It was not specified which allele was used.

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Present		3E left	Expressed in the early-born cortical neurons.

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Present		3E right	Expressed in the early-born cortical neurons, similar as in the wild-type.

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Present		3J left	Expressed in the early-born cortical neurons.

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Present		3J right	Significantly increased numbers of the positive early-born cortical neurons, compared with the wild-type. The proportion of BrdU+ early-born cortical neurons was significantly increased.

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Present		3N left	Expressed in the early-born cortical neurons.

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Present		3N right	Significantly increased numbers of the positive early-born cortical neurons, compared with the wild-type.
TS23: cerebral cortex intermediate zone	Present	Scattered	3N right	Mislocalization of the positive cells, indicating aberrant neuronal migration.

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Present			Expressed in the early-born cortical neurons, similar as in the wild-type.

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Present			Significantly increased numbers of the positive early-born cortical neurons. The proportion of BrdU+ early-born cortical neurons was significantly increased.

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Present			Significantly increased numbers of the positive early-born cortical neurons, compared with the wild-type. The proportion of BrdU+ early-born cortical neurons was significantly increased.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present		5A upper	Expressed in the neurons.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present		5A lower	Significant increase in the number of positive neurons.
TS26: cerebral cortex intermediate zone	Present		5A lower	A higher relative proportion of total positive neurons relative to the wild-type.
TS26: cortical plate	Present		5A lower	A lower proportion of total positive neurons relative to the wild-type.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present			Expressed in the neurons, similar as in the wild-type.
TS26: cerebral cortex intermediate zone	Present			A higher relative proportion of total positive neurons relative to the wild-type.
TS26: cortical plate	Present			A lower proportion of total positive neurons relative to the wild-type.

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Present		S2E left	Expressed in the early-born cortical neurons.

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Present		S2E right	Expressed in the early-born cortical neurons. The proportion of BrdU+ early-born cortical neurons was significantly increased.

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Present			Expressed in the early-born cortical neurons. The proportion of BrdU+ early-born cortical neurons was significantly increased.