Gene Expression Data
Assay Details
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Assay
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Reference:
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J:287484
Tomita H, et al., The Protein Tyrosine Phosphatase Receptor Delta Regulates Developmental Neurogenesis. Cell Rep. 2020 Jan 7;30(1):215-228.e5
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6437911
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Gene symbol:
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Eomes
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Gene name:
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eomesodermin
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Antibody:
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anti-TBR2/Eomes [EPR19012] (ab183991)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 555
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Assay notes:
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Another Ptprd knockout allele (Ptprd<tm2a(KOMP)Wtsi>) was mentioned in the paper. It was not specified which allele was used.
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Results
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Specimen
3C WT:
embryonic day 13.5
(more  )
Note: Double labeled: red - Eomes; green - BrdU. Quantified in Fig 3D, 3P.
Specimen
3C WT:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: red - Eomes; green - BrdU. Quantified in Fig 3D, 3P.
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Specimen
3C KO:
embryonic day 13.5; Ptprdtm1Yiw/ Ptprdtm1Yiw
(more )
Note: Double labeled: red - Eomes; green - BrdU. Quantified in Fig 3D, 3P.
Specimen
3C KO:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Ptprdtm1Yiw/Ptprdtm1Yiw
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: red - Eomes; green - BrdU. Quantified in Fig 3D, 3P.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cerebral cortex |
Present |
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3C right
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Modest increased numbers of the positive intermediate progenitors, compared with the wild-type. The proportion of BrdU+ intermediate progenitorswas significantly increased.
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Specimen
3H WT:
embryonic day 14.5
(more )
Note: Double labeled: red - Eomes; green - BrdU. Quantified in Fig 3I, 3P.
Specimen
3H WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: red - Eomes; green - BrdU. Quantified in Fig 3I, 3P.
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Specimen
3H KO:
embryonic day 14.5; Ptprdtm1Yiw/ Ptprdtm1Yiw
(more )
Note: Double labeled: red - Eomes; green - BrdU. Quantified in Fig 3I, 3P.
Specimen
3H KO:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Ptprdtm1Yiw/Ptprdtm1Yiw
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: red - Eomes; green - BrdU. Quantified in Fig 3I, 3P.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex |
Present |
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3H right
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Significantly increased numbers of the positive intermediate progenitors, compared with the wild-type. The proportion of BrdU+ intermediate progenitorswas significantly increased.
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Specimen
3M WT:
embryonic day 15.5
(more )
Note: Quantified in Fig 3P.
Specimen
3M WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Quantified in Fig 3P.
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Specimen
3M KO:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Ptprdtm1Yiw/Ptprdtm1Yiw
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Quantified in Fig 3P.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
Present |
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3M right
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Significantly increased numbers of the positive intermediate progenitors, compared with the wild-type.
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Specimen
not shown:
embryonic day 13.5; Ptprdtm1Yiw/Ptprd +
(more )
Note: Double labeled: red - Eomes; green - BrdU. Quantified in Fig 3D, 3P.
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Ptprdtm1Yiw/Ptprd+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: red - Eomes; green - BrdU. Quantified in Fig 3D, 3P.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cerebral cortex |
Present |
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|
Significantly increased numbers of the positive intermediate progenitors, compared with the wild-type. The proportion of BrdU+ intermediate progenitorswas significantly increased.
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Specimen
not shown:
embryonic day 14.5; Ptprdtm1Yiw/Ptprd +
(more )
Note: Double labeled: red - Eomes; green - BrdU. Quantified in Fig 3I, 3P.
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Ptprdtm1Yiw/Ptprd+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: red - Eomes; green - BrdU. Quantified in Fig 3I, 3P.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex |
Present |
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Significantly increased numbers of the positive intermediate progenitors, compared with the wild-type. The proportion of BrdU+ intermediate progenitorswas significantly increased.
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Specimen
not shown:
embryonic day 15.5; Ptprdtm1Yiw/Ptprd +
(more )
Note: Quantified in Fig 3P.
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Ptprdtm1Yiw/Ptprd+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Quantified in Fig 3P.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
Present |
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Significantly increased numbers of the positive intermediate progenitors, compared with the wild-type.
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Specimen
S2C WT:
embryonic day 15.5
(more )
Note: Double labeled: red - Eomes; green - BrdU. Quantified in Fig S2D.
Specimen
S2C WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: red - Eomes; green - BrdU. Quantified in Fig S2D.
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Specimen
S2C KO:
embryonic day 15.5; Ptprdtm1Yiw/ Ptprdtm1Yiw
(more )
Note: Double labeled: red - Eomes; green - BrdU. Quantified in Fig S2D.
Specimen
S2C KO:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Ptprdtm1Yiw/Ptprdtm1Yiw
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: red - Eomes; green - BrdU. Quantified in Fig S2D.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
Present |
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S2C right
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Expressed in the intermediate progenitors. The proportion of BrdU+ intermediate progenitorswas significantly increased.
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Specimen
not shown:
embryonic day 15.5; Ptprdtm1Yiw/Ptprd +
(more )
Note: Double labeled: red - Eomes; green - BrdU. Quantified in Fig S2D.
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Ptprdtm1Yiw/Ptprd+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: red - Eomes; green - BrdU. Quantified in Fig S2D.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
Present |
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Expressed in the intermediate progenitors. The proportion of BrdU+ intermediate progenitorswas significantly increased.
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