Hoxa13 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:287563 Mercader N, et al., Ectopic Meis1 expression in the mouse limb bud alters P-D patterning in a Pbx1-independent manner. Int J Dev Biol. 2009;53(8-10):1483-94
Assay type:	RNA in situ
MGI Accession ID:	MGI:6436624
Gene symbol:	Hoxa13
Gene name:	homeobox A13

Probe:	Hoxa13 probe14
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 1M: embryonic day 9.5 (more

)
Note: 28 somite-stage embryo

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Absent		1M

Specimen 1N: embryonic day 10.0 (more

)
Note: 33 somite-stage embryo

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present	Regionally restricted	1N	Expression is seen in a distal-posterior domain.

Specimen 1O: embryonic day 11.0 (more

)
Note: 44 somite-stage embryo

Structure	Level	Pattern	Image	Note
TS18: forelimb bud mesenchyme	Present	Regionally restricted	1O	Expression is seen in a distal domain.

Specimen 1P: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: hand	Present		1P

Specimen 2G: embryonic day 10.0 (more

)
Note: 34 somite-stage embryo; section through the posterior limb

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present	Regionally restricted	2G	Expression is seen in a distal domain.

Specimen 2H: embryonic day 10.0 (more

)
Note: 34 somite-stage embryo; section through the medial limb

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present	Regionally restricted	2H	Expression is seen in a distal domain.

Specimen 2I: embryonic day 10.0 (more

)
Note: 34 somite-stage embryo; section through the anterior limb

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present	Regionally restricted	2I	Expression is seen in a distal domain.

Specimen 6E: embryonic day 11.0 (more

)

Structure	Level	Pattern	Image	Note
TS18: forelimb bud mesenchyme	Present	Regionally restricted	6E	Expression is seen in a distal domain.

Specimen 6F: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: hand	Present		6F

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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