Antibody:	anti-cleaved caspase-3 (Asp175) (5A1E; #9664)
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS20: labyrinthine zone	Trace	Single cells	S4A WT E12.5	Only extremely rare positive cells were noted in the labyrinth.

Structure	Level	Pattern	Image	Note
TS20: labyrinthine zone	Trace	Single cells	S4A KO E12.5	Only extremely rare positive cells were noted in the labyrinth of the knockout as in the wild type.

Structure	Level	Pattern	Image	Note
TS23: labyrinthine zone	Trace	Single cells	S4A WT E15.5	Only extremely rare positive cells were noted in the labyrinth.

Structure	Level	Pattern	Image	Note
TS23: labyrinthine zone	Trace	Single cells	S4A KO E15.5	Only extremely rare positive cells were noted in the labyrinth of the knockout as in the wild type.