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Gene Expression Data
Assay Details
Assay
Reference: J:268201 Zhang Z, et al., Generation of MLC-2v-tdTomato knock-in reporter mouse line. Genesis. 2018 Oct;56(10):e23256
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6433831
Gene symbol: Myl2
Gene name: myosin, light polypeptide 2, regulatory, cardiac, slow
Antibody: Anti-MLC-2v
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 4b +/+: postnatal day 0; Myl2tm1.1Cya/Myl2tm1.1Cya (more )
Note: Triple labeled: yellow - Myl2; green - Tnnt2 (assay MGI:6433836); red - Myl2 (assay MGI:6433045).
Structure Level Pattern Image Note
TS27: heart ventricle Present 4b +/+ Merged , 4b +/+ MLC-2v Comparing immunostaining of the endogenous Myl2 with the tdTomato reporter indicates that there was no significant difference in the endogenous protein expression among the distinct genotypes.
Specimen 4b +/-: postnatal day 0; Myl2tm1.1Cya/Myl2+ (more )
Note: Triple labeled: yellow - Myl2; green - Tnnt2 (assay MGI:6433836); red - Myl2 (assay MGI:6433045).
Structure Level Pattern Image Note
TS27: heart ventricle Present 4b +/- MLC-2v , 4b +/- Merged Comparing immunostaining of the endogenous Myl2 with the tdTomato reporter indicates that there was no significant difference in the endogenous protein expression among the distinct genotypes.
Specimen 4b -/-: postnatal day 0 (more )
Note: Wild type. Double labeled: yellow - Myl2; green - Tnnt2 (assay MGI:6433836).
Structure Level Pattern Image Note
TS27: heart ventricle Present 4b -/- Merged , 4b -/- MLC-2v In the wild type (shown here as -/- for the reporter genotype), the sample shows endogenous protein level.
Specimen 4c +/+: postnatal week 6; Myl2tm1.1Cya/Myl2tm1.1Cya (more )
Note: Double labeled: yellow - Myl2; red - Myl2 (assay MGI:6433045).
Structure Level Pattern Image Note
TS28: heart ventricle Present 4c +/+ Merged bottom , 4c +/+ MLC-2v bottom , 4c +/+ Merged top , 4c +/+ MLC-2v top The adult homozygous mouse heart clearly demonstrated strong tdTomato expression in every ventricular cardiomyocyte expressing the endogenous Myl2.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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