About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:268201 Zhang Z, et al., Generation of MLC-2v-tdTomato knock-in reporter mouse line. Genesis. 2018 Oct;56(10):e23256
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6433831
Gene symbol: Myl2
Gene name: myosin, light polypeptide 2, regulatory, cardiac, slow
Antibody: Anti-MLC-2v
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 4b +/+: postnatal day 0; Myl2tm1.1Cya/Myl2tm1.1Cya (more )
Note: Triple labeled: yellow - Myl2; green - Tnnt2 (assay MGI:6433836); red - Myl2 (assay MGI:6433045).
Structure Level Pattern Image Note
TS27: heart ventricle Present 4b +/+ Merged , 4b +/+ MLC-2v Comparing immunostaining of the endogenous Myl2 with the tdTomato reporter indicates that there was no significant difference in the endogenous protein expression among the distinct genotypes.
Specimen 4b +/-: postnatal day 0; Myl2tm1.1Cya/Myl2+ (more )
Note: Triple labeled: yellow - Myl2; green - Tnnt2 (assay MGI:6433836); red - Myl2 (assay MGI:6433045).
Structure Level Pattern Image Note
TS27: heart ventricle Present 4b +/- MLC-2v , 4b +/- Merged Comparing immunostaining of the endogenous Myl2 with the tdTomato reporter indicates that there was no significant difference in the endogenous protein expression among the distinct genotypes.
Specimen 4b -/-: postnatal day 0 (more )
Note: Wild type. Double labeled: yellow - Myl2; green - Tnnt2 (assay MGI:6433836).
Structure Level Pattern Image Note
TS27: heart ventricle Present 4b -/- Merged , 4b -/- MLC-2v In the wild type (shown here as -/- for the reporter genotype), the sample shows endogenous protein level.
Specimen 4c +/+: postnatal week 6; Myl2tm1.1Cya/Myl2tm1.1Cya (more )
Note: Double labeled: yellow - Myl2; red - Myl2 (assay MGI:6433045).
Structure Level Pattern Image Note
TS28: heart ventricle Present 4c +/+ Merged bottom , 4c +/+ MLC-2v bottom , 4c +/+ Merged top , 4c +/+ MLC-2v top The adult homozygous mouse heart clearly demonstrated strong tdTomato expression in every ventricular cardiomyocyte expressing the endogenous Myl2.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/28/2024
MGI 6.13 		The Jackson Laboratory