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Gene Expression Data
Assay Details
Assay
Reference: J:181884 Quinn ME, et al., Preaxial polydactyly caused by Gli3 haploinsufficiency is rescued by Zic3 loss of function in mice. Hum Mol Genet. 2012 Apr 15;21(8):1888-96
Assay type: RT-PCR
MGI Accession ID: MGI:6430380
Gene symbol: Shh
Gene name: sonic hedgehog
Probe: Shh-pO3, Shh-pP3
Probe preparation: labelled with SYBR green
Assay notes: Posterior hindlimb. Quantitative RT-PCR. Relative expression to wild-type is shown normalized to Gapdh expression.
Results Image: 5
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
WT E11.5 TS19: hindlimb bud Present Not Specified; poly-A+ RNA involves: 129S7/SvEvBrd * C3H/HeJ * C57BL/6J Not Specified
Zic3 null E11.5 TS19: hindlimb bud Present (a) Not Specified; poly-A+ RNA involves: 129S7/SvEvBrd * C3H/HeJ * C57BL/6J Zic3tm1Bca/Zic3tm1BcaNot Specified
Gli3+/- E11.5 TS19: hindlimb bud Present (b) Not Specified; poly-A+ RNA involves: 129S7/SvEvBrd * C3H/HeJ * C57BL/6J Gli3Xt-J/Gli3+Not Specified
Zic3 null;Gli3+/- E11.5 TS19: hindlimb bud Present (c) Not Specified; poly-A+ RNA involves: 129S7/SvEvBrd * C3H/HeJ * C57BL/6J Gli3Xt-J/Gli3+, Zic3tm1Bca/Zic3tm1BcaNot Specified
Gli3-/- E11.5 TS19: hindlimb bud Present (d) Not Specified; poly-A+ RNA involves: 129S7/SvEvBrd * C3H/HeJ * C57BL/6J Gli3Xt-J/Gli3Xt-JNot Specified
Zic3 null;Gli3-/- E11.5 TS19: hindlimb bud Present (c) Not Specified; poly-A+ RNA involves: 129S7/SvEvBrd * C3H/HeJ * C57BL/6J Gli3Xt-J/Gli3Xt-J, Zic3tm1Bca/Zic3tm1BcaNot Specified
Notes:
(a) Expression increased 2.7-fold compared to wild-type.
(b) Expression increased 11-fold compared to wild-type.
(c) Expression is comparible to wild-type.
(d) Expression increased 6-fold compared to wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory