Shh RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:181884 Quinn ME, et al., Preaxial polydactyly caused by Gli3 haploinsufficiency is rescued by Zic3 loss of function in mice. Hum Mol Genet. 2012 Apr 15;21(8):1888-96
Assay type:	RNA in situ
MGI Accession ID:	MGI:6430372
Gene symbol:	Shh
Gene name:	sonic hedgehog

Probe:	Shh cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 4A: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	4A	Expression is posterior.

Specimen 4B: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud mesenchyme	Present	Regionally restricted	4B	Expression is posterior.

Specimen 4C: embryonic day 10.5; Zic3^tm1Bca/Zic3^tm1Bca (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	4C	Expression in this mutant is the same as in wild-type.

Specimen 4D: embryonic day 10.5; Zic3^tm1Bca/Zic3^tm1Bca (more

)

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud mesenchyme	Present	Regionally restricted	4D	Expression in this mutant is the same as in wild-type.

Specimen 4E: embryonic day 10.5; Gli3^Xt-J/Gli3^Xt-J (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	4E	Expression is posterior and there is ectopic expression at the anterior end.

Specimen 4F: embryonic day 10.5; Gli3^Xt-J/Gli3^Xt-J (more

)

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud mesenchyme	Present	Regionally restricted	4F	Expression is posterior and there is ectopic expression at the anterior end.

Specimen 4G: embryonic day 10.5; Gli3^Xt-J/Gli3^Xt-J, Zic3^tm1Bca/Zic3^tm1Bca (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	4G	Expression in this mutant is the same as in wild-type.

Specimen 4H: embryonic day 10.5; Gli3^Xt-J/Gli3^Xt-J, Zic3^tm1Bca/Zic3^tm1Bca (more

)

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud mesenchyme	Present	Regionally restricted	4H	Expression in this mutant is the same as in wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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