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Gene Expression Data
Assay Details
Assay
Reference: J:93163 Boulet AM, et al., The roles of Fgf4 and Fgf8 in limb bud initiation and outgrowth. Dev Biol. 2004 Sep 15;273(2):361-72
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6423485
Gene symbol: Fgf8
Gene name: fibroblast growth factor 8
Reporter: GFP
Antibody: anti-GFP (Invitrogen)
Detection system: Alexa Fluor 488
Results
Specimen 1C: embryonic day 9.0; Fgf8tm1Moon/Fgf8+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS14: intermediate mesenchyme Present 1C Authors state GFP expression reproduces the Fgf8 expression pattern.
Specimen 1D: embryonic day 9.0; Fgf8tm1Moon/Fgf8+, Tg(Lefty2-cre)21bHmd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS14: intermediate mesenchyme Present 1D Expression only seen where Fgf8 and Lefty2-Cre expression patterns overlap.
Specimen 1E: embryonic day 8.75; Fgf8tm1Moon/Fgf8+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS14: intermediate mesenchyme Present 1E Authors state GFP expression reproduces the Fgf8 expression pattern.
Specimen 1F: embryonic day 8.75; Fgf8tm1Moon/Fgf8+, Tg(Lefty2-cre)21bHmd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS13: intermediate mesenchyme Present 1F Expression only seen where Fgf8 and Lefty2-Cre expression patterns overlap.
Specimen 4A: embryonic day 9.5; Fgf8tm1Moon/Fgf8+, Tfap2atm1(cre)Moon/Tfap2a+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: forelimb bud ectoderm Present Regionally restricted 4A Expression in early forelimb bud ventral ectoderm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/06/2026
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