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Gene Expression Data
Assay Details
Assay
Reference: J:272566 Yang R, et al., CDK5RAP3, a UFL1 substrate adaptor, is crucial for liver development. Development. 2019 Jan 25;146(2):dev169235
Assay type: Western blot
MGI Accession ID: MGI:6423439
Gene symbol: Cdk5rap3
Gene name: CDK5 regulatory subunit associated protein 3
Antibody: Anti-CDK5RAP3 (H-300; sc-134627)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Two antibodies were described MGI:6423118 and MGI:6423120, but the one used in this assay was not specified. Either antibody could have been used.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Genetic BackgroundMutant Allele(s)Sex Note
Con P m 1 TS28: liver Present Not Specified Not Specified The genetic background of the conditional control specimen was not specified.
Con P m 1 TS28: liver Present Not Specified Not Specified The genetic background of the conditional control specimen was not specified.
Con P m 1 TS28: liver Present Not Specified Not Specified The genetic background of the conditional control specimen was not specified.
Con P m 1 TS28: liver Present Not Specified Not Specified The genetic background of the conditional control specimen was not specified.
CKO P m 1 TS28: liver Weak (a) involves: 129S4/SvJaeSor * C57BL/6N * DBA Cdk5rap3tm1c(EUCOMM)Hmgu/Cdk5rap3tm1c(EUCOMM)Hmgu, Tg(Foxa3-cre)1Khk/0Not Specified Conditional mutant.
CKO P m 1 TS28: liver Weak (a) involves: 129S4/SvJaeSor * C57BL/6N * DBA Cdk5rap3tm1c(EUCOMM)Hmgu/Cdk5rap3tm1c(EUCOMM)Hmgu, Tg(Foxa3-cre)1Khk/0Not Specified Conditional mutant.
CKO P m 1 TS28: liver Ambiguous involves: 129S4/SvJaeSor * C57BL/6N * DBA Cdk5rap3tm1c(EUCOMM)Hmgu/Cdk5rap3tm1c(EUCOMM)Hmgu, Tg(Foxa3-cre)1Khk/0Not Specified Conditional mutant.
CKO P m 1 TS28: liver Ambiguous involves: 129S4/SvJaeSor * C57BL/6N * DBA Cdk5rap3tm1c(EUCOMM)Hmgu/Cdk5rap3tm1c(EUCOMM)Hmgu, Tg(Foxa3-cre)1Khk/0Not Specified Conditional mutant.
Notes:
(a) There was a dramatic decrease in the level of Cdk5rap3 protein in the conditional knockout.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/29/2024
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