Hgd In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:287389 Hughes JH, et al., Conditional targeting in mice reveals that hepatic homogentisate 1,2-dioxygenase activity is essential in reducing circulating homogentisic acid and for effective therapy in the genetic disease alkaptonuria. Hum Mol Genet. 2019 Dec 1;28(23):3928-3939
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:6423321
Gene symbol:	Hgd
Gene name:	homogentisate 1, 2-dioxygenase

Reporter:	lacZ
	Direct Detection

Results

3A Hgd tm1a -/- 2h (upper) ©

Specimen 3A Hgd tm1a -/- 2 hr (upper): postnatal adult; Hgd^{tm1a(KOMP)Wtsi}/Hgd^{tm1a(KOMP)Wtsi} (more

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Structure	Level	Pattern	Image	Note
TS28: liver parenchyma	Present		3A Hgd tm1a -/- 2h (upper)

3A Hgd tm1a -/- Overnight (upper) ©

Specimen 3A Hgd tm1a -/- Overnight (upper): postnatal adult; Hgd^{tm1a(KOMP)Wtsi}/Hgd^{tm1a(KOMP)Wtsi} (more

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Structure	Level	Pattern	Image	Note
TS28: liver parenchyma	Strong		3A Hgd tm1a -/- Overnight (upper)

3A Hgd tm1a -/- 2h (lower) ©

Specimen 3A Hgd tm1a -/- 2 hr (lower): postnatal adult; Hgd^{tm1a(KOMP)Wtsi}/Hgd^{tm1a(KOMP)Wtsi} (more

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Structure	Level	Pattern	Image	Note
TS28: renal cortex	Present		3A Hgd tm1a -/- 2h (lower)

3A Hgd tm1a -/- Overnight (lower) ©

Specimen 3A Hgd tm1a -/- Overnight (lower): postnatal adult; Hgd^{tm1a(KOMP)Wtsi}/Hgd^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: renal cortex	Strong		3A Hgd tm1a -/- Overnight (lower)

Specimen heterozygous (not shown): postnatal adult; Hgd^{tm1a(KOMP)Wtsi}/Hgd⁺ (more

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Structure	Level	Pattern	Image	Note
TS28: liver parenchyma	Weak			Expression was less intense and slower to develop.
TS28: renal cortex	Weak			Expression was less intense and slower to develop.

Specimen all other tissues (not shown): postnatal adult; Hgd^{tm1a(KOMP)Wtsi}/Hgd^{tm1a(KOMP)Wtsi} (more

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Structure	Level	Pattern	Image	Note
TS28: bone	Absent
TS28: brain	Absent
TS28: cartilage	Absent
TS28: eye	Absent
TS28: heart	Absent
TS28: intestine	Absent
TS28: lung	Absent
TS28: muscle tissue	Absent
TS28: prostate gland	Absent
TS28: skin	Absent
TS28: spleen	Absent

3C ©

Specimen 3C: postnatal adult; Hgd^{tm1a(KOMP)Wtsi}/Hgd^{tm1a(KOMP)Wtsi} (more

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Structure	Level	Pattern	Image	Note
TS28: liver parenchyma	Present	Widespread	3C	Expression in cytoplasm throughout liver parenchyma.

3D ©

Specimen 3D: postnatal adult; Hgd^{tm1a(KOMP)Wtsi}/Hgd^{tm1a(KOMP)Wtsi} (more

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Structure	Level	Pattern	Image	Note
TS28: renal cortex	Present	Regionally restricted	3D	Expression only in certain tubules.

3E ©

Specimen 3E: postnatal adult; Hgd^{tm1a(KOMP)Wtsi}/Hgd^{tm1a(KOMP)Wtsi} (more

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Structure	Level	Pattern	Image	Note
TS28: glomerular tuft	Absent		3E	No expression in glomeruli.

3F ©

Specimen 3F: postnatal adult; Hgd^{tm1a(KOMP)Wtsi}/Hgd^{tm1a(KOMP)Wtsi} (more

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Structure	Level	Pattern	Image	Note
TS28: renal proximal convoluted tubule	Present	Regionally restricted	3F	Expression coincides with PAS-positive cells in brush border of proximal convoluted tubules.

3G E13.5 ©

Specimen 3G E13.5: embryonic day 13.5; Hgd^{tm1a(KOMP)Wtsi}/Hgd^{tm1a(KOMP)Wtsi} (more

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Structure	Level	Pattern	Image	Note
TS21: embryo	Absent		3G E13.5

3G E14.5 ©

Specimen 3G E14.5: embryonic day 14.5; Hgd^{tm1a(KOMP)Wtsi}/Hgd^{tm1a(KOMP)Wtsi} (more

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Structure	Level	Pattern	Image	Note
TS22: liver	Present		3G E14.5	Expression in liver from E14.5 onwards.

3G E15.5 ©

Specimen 3G E15.5: embryonic day 15.5; Hgd^{tm1a(KOMP)Wtsi}/Hgd^{tm1a(KOMP)Wtsi} (more

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Structure	Level	Pattern	Image	Note
TS23: liver	Present		3G E15.5	Expression in liver from E14.5 onwards.

Specimen 3H E11.5 Liver: embryonic day 11.5; Hgd^{tm1a(KOMP)Wtsi}/Hgd^{tm1a(KOMP)Wtsi} (more

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Structure	Level	Pattern	Image	Note
TS19: liver	Absent		3H E11.5 Liver

Specimen 3H E12.5 Liver: embryonic day 12.5; Hgd^{tm1a(KOMP)Wtsi}/Hgd^{tm1a(KOMP)Wtsi} (more

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Structure	Level	Pattern	Image	Note
TS20: liver	Present		3H E12.5 Liver

Specimen 3H E13.5 Liver: embryonic day 13.5; Hgd^{tm1a(KOMP)Wtsi}/Hgd^{tm1a(KOMP)Wtsi} (more

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Structure	Level	Pattern	Image	Note
TS22: liver	Present		3H E13.5 Liver

Specimen 3H E13.5 Kidney: embryonic day 13.5; Hgd^{tm1a(KOMP)Wtsi}/Hgd^{tm1a(KOMP)Wtsi} (more

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Structure	Level	Pattern	Image	Note
TS22: metanephros	Absent		3H E13.5 Kidney

Specimen 3H E14.5 Kidney: embryonic day 14.5; Hgd^{tm1a(KOMP)Wtsi}/Hgd^{tm1a(KOMP)Wtsi} (more

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Structure	Level	Pattern	Image	Note
TS22: metanephros	Absent		3H E14.5 Kidney

Specimen 3H E15.5 Kidney: embryonic day 15.5; Hgd^{tm1a(KOMP)Wtsi}/Hgd^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS23: metanephros	Present		3H E15.5 Kidney

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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