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Gene Expression Data
Assay Details
Assay
Reference: J:199301 Lavado A, et al., Tumor suppressor Nf2 limits expansion of the neural progenitor pool by inhibiting Yap/Taz transcriptional coactivators. Development. 2013 Aug;140(16):3323-34
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6416434
Gene symbol: Gfap
Gene name: glial fibrillary acidic protein
Results
Specimen 5O: embryonic day 17.5 (more )
Note: The genetic background of the conditional control specimen was not specified. Double labeled: red - Gfap; green - Reln (assay MGI:6416432).
Structure Level Pattern Image Note
TS25: telencephalon ventricular layer Present Regionally restricted 5O Immunopositive fibers extended along the cortical hem-dentate neuroepithelium boundary and into the subpial space.
TS25: hippocampus Present Regionally restricted 5O The fibers from the cortical hem-dentate neuroepithelium boundary and the subpial space were underneath the Cajal Retzius cells at the hippocampal fissure.
Specimen 5P: embryonic day 17.5; Nf2tm2Gth/Nf2tm2Gth, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Gfap; green - Reln (assay MGI:6416432).
Structure Level Pattern Image Note
TS25: telencephalon ventricular layer Present Regionally restricted 5P Immunopositive fibers became overabundant but severly disorganized; the fibers were not restricted to the cortical hem-dentate neuroepithelium boundary and the subpial space. Fibers were also extended in much of the cortical hem.
TS25: hippocampus Present Regionally restricted 5P Immunopositive fibers were in a more extended region encompassing much of the hippocampal primordium.
Specimen 8S: postnatal day 0; Nf2tm2Gth/Nf2+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Control specimen. Double labeled: red - Gfap; green - Reln (assay MGI:6416432).
Structure Level Pattern Image Note
TS27: dentate gyrus Present Regionally restricted 8S Expression was detected in the dentate glial scaffold.
Specimen 8T: postnatal day 0; Nf2tm2Gth/Nf2tm2Gth, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Gfap; green - Reln (assay MGI:6416432).
Structure Level Pattern Image Note
TS27: dentate gyrus Present Regionally restricted 8T Defects were noted in the dentate glial scaffold.
TS27: allocortex Present Regionally restricted 8T The mutant demonstrated excessive Gfap+ fibers at the cortical hem, and the absence of immunopositive glial fibers below the Reln+ hippocampal fissure.
Specimen 8U: postnatal day 0; Nf2tm2Gth/Nf2+, Yap1tm1.1Eno/Yap1tm1.1Eno, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Gfap; green - Reln (assay MGI:6416432).
Structure Level Pattern Image Note
TS27: dentate gyrus Present 8U
Specimen 8V: postnatal day 0; Nf2tm2Gth/Nf2tm2Gth, Yap1tm1.1Eno/Yap1+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Gfap; green - Reln (assay MGI:6416432).
Structure Level Pattern Image Note
TS27: dentate gyrus Present 8V
Specimen 8W: postnatal day 0; Nf2tm2Gth/Nf2tm2Gth, Yap1tm1.1Eno/Yap1tm1.1Eno, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Gfap; green - Reln (assay MGI:6416432).
Structure Level Pattern Image Note
TS27: dentate gyrus Present Regionally restricted 8W In six out of ten Nf2<F/F>;Yap<F/F>;Emx1-Cre hippocampi, the radial glial scaffold appeared identical to that in control (8S) brains.
Specimen S9D: postnatal day 0; Nf2tm2Gth/Nf2tm2Gth, Yap1tm1.1Eno/Yap1tm1.1Eno, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Gfap; green - Reln (assay MGI:6416432).
Structure Level Pattern Image Note
TS27: dentate gyrus Present Regionally restricted S9D In four out of ten Nf2<F/F>;Yap<F/F>;Emx1-Cre hippocampi, the radial glial scaffold showed partial rescue of the defects due to the Nf2 mutation.
TS27: allocortex Present Regionally restricted S9D Ectopic fibers were still found at the cortical hem.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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