About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:199301 Lavado A, et al., Tumor suppressor Nf2 limits expansion of the neural progenitor pool by inhibiting Yap/Taz transcriptional coactivators. Development. 2013 Aug;140(16):3323-34
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6416434
Gene symbol: Gfap
Gene name: glial fibrillary acidic protein
Results
Specimen 5O: embryonic day 17.5 (more )
Note: The genetic background of the conditional control specimen was not specified. Double labeled: red - Gfap; green - Reln (assay MGI:6416432).
Structure Level Pattern Image Note
TS25: telencephalon ventricular layer Present Regionally restricted 5O Immunopositive fibers extended along the cortical hem-dentate neuroepithelium boundary and into the subpial space.
TS25: hippocampus Present Regionally restricted 5O The fibers from the cortical hem-dentate neuroepithelium boundary and the subpial space were underneath the Cajal Retzius cells at the hippocampal fissure.
Specimen 5P: embryonic day 17.5; Nf2tm2Gth/Nf2tm2Gth, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Gfap; green - Reln (assay MGI:6416432).
Structure Level Pattern Image Note
TS25: telencephalon ventricular layer Present Regionally restricted 5P Immunopositive fibers became overabundant but severly disorganized; the fibers were not restricted to the cortical hem-dentate neuroepithelium boundary and the subpial space. Fibers were also extended in much of the cortical hem.
TS25: hippocampus Present Regionally restricted 5P Immunopositive fibers were in a more extended region encompassing much of the hippocampal primordium.
Specimen 8S: postnatal day 0; Nf2tm2Gth/Nf2+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Control specimen. Double labeled: red - Gfap; green - Reln (assay MGI:6416432).
Structure Level Pattern Image Note
TS27: dentate gyrus Present Regionally restricted 8S Expression was detected in the dentate glial scaffold.
Specimen 8T: postnatal day 0; Nf2tm2Gth/Nf2tm2Gth, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Gfap; green - Reln (assay MGI:6416432).
Structure Level Pattern Image Note
TS27: dentate gyrus Present Regionally restricted 8T Defects were noted in the dentate glial scaffold.
TS27: allocortex Present Regionally restricted 8T The mutant demonstrated excessive Gfap+ fibers at the cortical hem, and the absence of immunopositive glial fibers below the Reln+ hippocampal fissure.
Specimen 8U: postnatal day 0; Nf2tm2Gth/Nf2+, Yap1tm1.1Eno/Yap1tm1.1Eno, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Gfap; green - Reln (assay MGI:6416432).
Structure Level Pattern Image Note
TS27: dentate gyrus Present 8U
Specimen 8V: postnatal day 0; Nf2tm2Gth/Nf2tm2Gth, Yap1tm1.1Eno/Yap1+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Gfap; green - Reln (assay MGI:6416432).
Structure Level Pattern Image Note
TS27: dentate gyrus Present 8V
Specimen 8W: postnatal day 0; Nf2tm2Gth/Nf2tm2Gth, Yap1tm1.1Eno/Yap1tm1.1Eno, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Gfap; green - Reln (assay MGI:6416432).
Structure Level Pattern Image Note
TS27: dentate gyrus Present Regionally restricted 8W In six out of ten Nf2<F/F>;Yap<F/F>;Emx1-Cre hippocampi, the radial glial scaffold appeared identical to that in control (8S) brains.
Specimen S9D: postnatal day 0; Nf2tm2Gth/Nf2tm2Gth, Yap1tm1.1Eno/Yap1tm1.1Eno, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Gfap; green - Reln (assay MGI:6416432).
Structure Level Pattern Image Note
TS27: dentate gyrus Present Regionally restricted S9D In four out of ten Nf2<F/F>;Yap<F/F>;Emx1-Cre hippocampi, the radial glial scaffold showed partial rescue of the defects due to the Nf2 mutation.
TS27: allocortex Present Regionally restricted S9D Ectopic fibers were still found at the cortical hem.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/14/2024
MGI 6.23 		The Jackson Laboratory