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Gene Expression Data
Assay Details
Assay
Reference: J:199301 Lavado A, et al., Tumor suppressor Nf2 limits expansion of the neural progenitor pool by inhibiting Yap/Taz transcriptional coactivators. Development. 2013 Aug;140(16):3323-34
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6416322
Gene symbol: Prox1
Gene name: prospero homeobox 1
Antibody: anti-Prox1 (AF2727)
Assay notes: Two antibodies were described MGI:5587807 and MGI:5431536, but the one used in this assay was not specified. Either antibody could have been used.
Results
Specimen 4E: embryonic day 14.5 (more )
Note: The genetic background of the conditional control specimen was not specified.
Structure Level Pattern Image Note
TS22: telencephalon ventricular layer Present Regionally restricted 4E Expression was detected in the dentate neuroepithelium.
Specimen 4F: embryonic day 14.5; Nf2tm2Gth/Nf2tm2Gth, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: telencephalon ventricular layer Present Regionally restricted 4F Normal expression was detected in the dentate neuroepithelium of the conditional mutant.
Specimen 4M: embryonic day 17.5 (more )
Note: The genetic background of the conditional control specimen was not specified.
Structure Level Pattern Image Note
TS25: dentate gyrus Present 4M
Specimen 4N: embryonic day 17.5; Nf2tm2Gth/Nf2tm2Gth, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS25: dentate gyrus Present 4N In the mutant, the region occupied by postmitotic neurons was much smaller.
Specimen 5K: embryonic day 17.5 (more )
Note: The genetic background of the conditional control specimen was not specified. Double labeled: red - Prox1; green - Reln (assay MGI:6416432).
Structure Level Pattern Image Note
TS25: dentate gyrus Present 5K
Specimen 5L: embryonic day 17.5; Nf2tm2Gth/Nf2tm2Gth, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Prox1; green - Reln (assay MGI:6416432).
Structure Level Pattern Image Note
TS25: dentate gyrus Present 5L
Specimen 5M: embryonic day 17.5 (more )
Note: The genetic background of the conditional control specimen was not specified. Double labeled: red - Prox1; green - Calb2 (assay MGI:6416433).
Structure Level Pattern Image Note
TS25: dentate gyrus Present 5M
Specimen 5N: embryonic day 17.5; Nf2tm2Gth/Nf2tm2Gth, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Prox1; green - Calb2 (assay MGI:6416433).
Structure Level Pattern Image Note
TS25: dentate gyrus Present 5N
Specimen 8D: postnatal day 0; Nf2tm2Gth/Nf2+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: dentate gyrus Present 8D
Specimen 8E: postnatal day 0; Nf2tm2Gth/Nf2tm2Gth, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: dentate gyrus Present 8E
Specimen 8F: postnatal day 0; Nf2tm2Gth/Nf2+, Yap1tm1.1Eno/Yap1tm1.1Eno, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: dentate gyrus Present 8F
Specimen 8G: postnatal day 0; Nf2tm2Gth/Nf2tm2Gth, Yap1tm1.1Eno/Yap1+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: dentate gyrus Present 8G
Specimen 8H: postnatal day 0; Nf2tm2Gth/Nf2tm2Gth, Yap1tm1.1Eno/Yap1tm1.1Eno, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: dentate gyrus Present 8H In six out of ten Nf2<F/F>;Yap<F/F>;Emx1-Cre hippocampi, the dentate gyrus appeared identical to those in control (8D) brains.
Specimen S9A: postnatal day 0; Nf2tm2Gth/Nf2tm2Gth, Yap1tm1.1Eno/Yap1tm1.1Eno, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: dentate gyrus Present S9A In four out of ten Nf2<F/F>;Yap<F/F>;Emx1-Cre hippocampi, the dentate gyrus with distinguished double blades showed partial rescue of defects due to the Nf2 mutation.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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