Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:286994
Chomphoo S, et al., Discrete localization patterns of Arf6, and its activators EFA6A and BRAG2, and its effector PIP5kinasegamma on myofibrils of myotubes and plasma membranes of myoblasts in developing skeletal muscles of mice. Acta Histochem. 2020 Apr;122(3):151513
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:6416291
|
Gene symbol:
|
Iqsec1
|
Gene name:
|
IQ motif and Sec7 domain 1
|
Antibody:
|
BRAG2 antibody
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
Results
|
Specimen
2d:
embryonic day 18.0
(more )
Specimen
2d:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: forelimb muscle |
Ambiguous |
Regionally restricted |
2d
|
Authors state striations are not signficantly immunoreactive for BRAG2.
|
Specimen
4e:
embryonic day 14.0
(more )
Specimen
4e:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 14.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
4f:
embryonic day 14.0
(more )
Specimen
4f:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 14.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: forelimb muscle |
Present |
Regionally restricted |
4f
|
Expression in plasmalemma of myoblasts.
|
|