Antibody:	anti-PGP9.5
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: enteric nervous system	Present	Regionally restricted	2A	Expressed as a complete ring in enteric neurons.
TS20: intestine mesenchyme	Present	Regionally restricted	2A	Expressed as a complete ring in enteric neurons.

Structure	Level	Pattern	Image	Note
TS20: enteric nervous system	Present	Scattered	2B	Only sporadic positve cells were observed.
TS20: intestine mesenchyme	Present	Scattered	2B	Only sporadic positve cells were observed.

Structure	Level	Pattern	Image	Note
TS22: enteric nervous system	Present	Clusters	2C	Positive cells coalesced as the enteric nervous system matured and ganglia began to form.
TS22: intestine mesenchyme	Present	Clusters	2C	Positive cells coalesced as the enteric nervous system matured and ganglia began to form.

Structure	Level	Pattern	Image	Note
TS22: enteric nervous system	Present	Scattered	2D	Only sporadic positve cells were observed.
TS22: intestine mesenchyme	Present	Scattered	2D	Only sporadic positve cells were observed.

Structure	Level	Pattern	Image	Note
TS24: enteric nervous system	Present	Clusters	2E	Positive cells coalesced as the enteric nervous system matured and ganglia began to form.
TS24: intestine mesenchyme	Present	Clusters	2E	Positive cells coalesced as the enteric nervous system matured and ganglia began to form.

Structure	Level	Pattern	Image	Note
TS24: enteric nervous system	Present	Scattered	2F	Only sporadic positve cells were observed.
TS24: intestine mesenchyme	Present	Scattered	2F	Only sporadic positve cells were observed.

Structure	Level	Pattern	Image	Note
TS19: enteric nervous system	Present	Regionally restricted	4C	Expressed as an incomplete ring in proximal intestinal loops, still scattered more distally.
TS19: intestine mesenchyme	Present	Regionally restricted	4C	Expressed as an incomplete ring in proximal intestinal loops, still scattered more distally.

Structure	Level	Pattern	Image	Note
TS19: enteric nervous system	Present	Regionally restricted	4D	Expressed only in the mesenchyme of the lateral wall of the stomach and not more distally in the gastrointestinal tract.
TS19: stomach mesenchyme	Present	Regionally restricted	4D	Expressed only in the mesenchyme of the lateral wall of the stomach and not more distally in the gastrointestinal tract.
TS19: intestine mesenchyme	Absent		4D