Antibody:	DGK-theta antibody (E-5)
Detection system:	horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	1A	Expression along surface of forebrain, rhombencephalon and neural tube.
TS17: hindbrain	Present	Regionally restricted	1A	Expression along surface of forebrain, rhombencephalon and neural tube.
TS17: neural tube	Present	Regionally restricted	1A	Expression along surface of forebrain, rhombencephalon and neural tube.
TS17: branchial arch	Present		1A
TS17: bulbus cordis	Present		1A
TS17: notochord	Present		1A
TS17: head mesenchyme	Weak		1A	Faint expression in mesenchymal cells around cephalic region.
TS17: diencephalon	Weak	Regionally restricted	1A	Faint expression in neuroepithelium surrounding third ventricle.
TS17: hindlimb bud ectoderm	Present		1A	Distinct expression in ectodermal epithelium of hindbud.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	1B	Expression along surface of rhombencephalon.
TS17: head mesenchyme	Weak	Regionally restricted	1B	Faint expression in mesenchymal cells around cephalic region.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	1C	Expression along surface of forebrain.
TS17: diencephalon	Weak	Regionally restricted	1C	Faint expression in neuroepithelium surrounding third ventricle.

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present		1D

Structure	Level	Pattern	Image	Note
TS17: bulbus cordis	Present		1E

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	1F	Expression along surface of neural tube.
TS17: notochord	Present		1F

Structure	Level	Pattern	Image	Note
TS17: hepatic primordium	Present		1G
TS17: midgut vascular element	Present	Regionally restricted	1G	Expression in midgut artery.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud ectoderm	Present		1H	Distinct expression in ectodermal epithelium of hindbud.

Structure	Level	Pattern	Image	Note
TS19: forebrain	Present		1I	Authors state a similar pattern of expression to that seen at E10.5 persisted at E11.5.
TS19: future spinal cord	Present		1I	Authors state a similar pattern of expression to that seen at E10.5 persisted at E11.5.
TS19: head mesenchyme	Present		1I	Authors state a similar pattern of expression to that seen at E10.5 persisted at E11.5.
TS19: tongue mesenchyme	Strong	Regionally restricted	1I	Relatively high expression in mesenchymal cells around periphery of tongue.
TS19: nasal mesenchyme	Strong	Regionally restricted	1I	Relatively high expression in mesenchymal cells around periphery of nasal placode.
TS19: bronchus	Present		1I
TS19: dorsal root ganglion	Present		1I
TS19: liver	Present		1I
TS19: stomach lumen	Present		1I

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present		1J

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Strong	Regionally restricted	1K	Expression in neuroepithelium surrounding third ventricle significantly increased compared to E10.5.

Structure	Level	Pattern	Image	Note
TS19: trachea	Present		1L

Structure	Level	Pattern	Image	Note
TS19: tongue	Present		1M

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		1O

Structure	Level	Pattern	Image	Note
TS19: liver	Present		1P

Structure	Level	Pattern	Image	Note
TS19: heart	Present	Regionally restricted	1Q	Expression in bulbus cordis.
TS19: midgut	Present		1Q

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	2A	Increased intensity of expression in cephalic region at rostral side of telencephalon, tectum of midbrain and pontine flexure.
TS20: midbrain	Present	Regionally restricted	2A	Increased intensity of expression in cephalic region at rostral side of telencephalon, tectum of midbrain and pontine flexure.
TS20: telencephalon	Present	Regionally restricted	2A	Increased intensity of expression in cephalic region at rostral side of telencephalon, tectum of midbrain and pontine flexure.
TS20: nasal cavity epithelium	Present	Regionally restricted	2A	Expression expanded to surface layer of nasal cavity.
TS20: heart	Present		2A	Expression persisted or increased in tongue, lung and heart.
TS20: lung	Present		2A	Expression persisted or increased in tongue, lung and heart.
TS20: tongue	Present		2A	Expression persisted or increased in tongue, lung and heart.
TS20: arterial system endothelium	Absent		2A	Expression undetectable in endothelial cells of arterial trunk.
TS20: dorsal root ganglion	Present		2A

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Strong	Regionally restricted	2B	Increased intensity of expression in tectum of midbrain and pontine flexure.
TS20: midbrain	Strong	Regionally restricted	2B	Increased intensity of expression in tectum of midbrain and pontine flexure.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Regionally restricted	2C	Increased intensity of expression in cephalic region at rostral side of telencephalon.

Structure	Level	Pattern	Image	Note
TS20: lung	Present		2D	Expression persisted or increased in lung.

Structure	Level	Pattern	Image	Note
TS20: dental placode	Present	Regionally restricted	2E	Expression expanded to dental lamina.

Structure	Level	Pattern	Image	Note
TS21: embryo	Present	Regionally restricted		Authors report a similar pattern of expression at E13.5.

Structure	Level	Pattern	Image	Note
TS22: brain	Present	Widespread	2F	Expression widely distributed in all brain regions.
TS22: epithalamus	Very strong		2F	Particularly dense expression in neocortex, epithalamus and medulla oblongata.
TS22: medulla oblongata	Very strong		2F	Particularly dense expression in neocortex, epithalamus and medulla oblongata.
TS22: neocortex	Very strong		2F	Particularly dense expression in neocortex, epithalamus and medulla oblongata.
TS22: epithelium of urogenital sinus	Strong		2F	Dense expression continually detected within various types of epithelia, including tongue, nasal cavity, midgut and urogenital sinus.
TS22: midgut epithelium	Strong		2F	Dense expression continually detected within various types of epithelia, including tongue, nasal cavity, midgut and urogenital sinus.
TS22: nasal cavity epithelium	Strong		2F	Dense expression continually detected within various types of epithelia, including tongue, nasal cavity, midgut and urogenital sinus.
TS22: tongue epithelium	Strong		2F	Dense expression continually detected within various types of epithelia, including tongue, nasal cavity, midgut and urogenital sinus.
TS22: arterial system endothelium	Absent		2F	Expression undetectable in endothelial cell of arterial trunk.

Structure	Level	Pattern	Image	Note
TS22: epithalamus	Strong		2G

Structure	Level	Pattern	Image	Note
TS22: neocortex	Strong		2H
TS22: ganglionic eminence	Absent		2H

Structure	Level	Pattern	Image	Note
TS22: tongue epithelium	Strong		2I	Dense expression continually detected within various types of epithelia, including tongue and tooth bud.
TS22: tooth bud	Strong		2I	Dense expression continually detected within various types of epithelia, including tongue and tooth bud.

Structure	Level	Pattern	Image	Note
TS22: nasal cavity epithelium	Strong		2J	Dense expression continually detected within various types of epithelia, including nasal cavity.
TS22: epidermis	Strong		2J	Significant increase in expression in squamous epithelial cells of skin, which successively differentiate into epidermis.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Very strong		2K	Particularly dense expression in medulla oblongata.

Structure	Level	Pattern	Image	Note
TS22: brain	Present	Widespread	2L	Expression widely distributed in all brain regions.

Structure	Level	Pattern	Image	Note
TS22: tongue epithelium	Strong		2M	Dense expression continually detected within various types of epithelia, including tongue.

Structure	Level	Pattern	Image	Note
TS22: spinal cord gray matter	Strong	Regionally restricted	2N	Particularly dense expression in gray horn of spinal cord.
TS22: epidermis	Strong	Regionally restricted	2N	Significant increase in expression in squamous epithelial cells of dorsal skin, which successively differentiate into epidermis.

Structure	Level	Pattern	Image	Note
TS22: midgut epithelium	Strong		2O	Dense expression continually detected within various types of epithelia, including midgut.

Structure	Level	Pattern	Image	Note
TS22: epithelium of urogenital sinus	Strong		2P	Dense expression continually detected within various types of epithelia, including urogenital sinus.

Structure	Level	Pattern	Image	Note
TS24: neocortex	Present		3A

Structure	Level	Pattern	Image	Note
TS24: lung	Weak		3B	Expression was attenuated in lung.

Structure	Level	Pattern	Image	Note
TS24: intestinal villus	Strong		3C	Expression increased in villi and mucosa of intestine.
TS24: intestine mucosa	Strong		3C	Expression increased in villi and mucosa of intestine.

Structure	Level	Pattern	Image	Note
TS24: back skin	Strong	Regionally restricted	3D	Expression increased in basal layer of dorsal skin.

Structure	Level	Pattern	Image	Note
TS25: brain	Present

Structure	Level	Pattern	Image	Note
TS26: embryo	Present	Regionally restricted	3E

Structure	Level	Pattern	Image	Note
TS26: neocortex	Present		3F

Structure	Level	Pattern	Image	Note
TS26: enamel epithelium	Weak	Regionally restricted	3G	Faint expression in enamel epithelium of incisor teeth.

Structure	Level	Pattern	Image	Note
TS26: epidermis	Weak	Regionally restricted	3H	Faint expression in epidermis of face.

Structure	Level	Pattern	Image	Note
TS26: main bronchus	Weak		3I
TS26: trachea	Weak		3I

Structure	Level	Pattern	Image	Note
TS26: heart ventricle wall	Weak		3J

Structure	Level	Pattern	Image	Note
TS26: epidermis	Weak		3K	Faint expression in epidermis of dorsal surfaces.

Structure	Level	Pattern	Image	Note
TS26: liver	Present		3L

Structure	Level	Pattern	Image	Note
TS26: intestinal villus	Strong		3M	Strong expression in villi and mucosa of intestine.
TS26: intestine mucosa	Strong		3M	Strong expression in villi and mucosa of intestine.

Structure	Level	Pattern	Image	Note
TS25: stomach	Present	Regionally restricted	S1Aa	Expression in cardiac end of stomach.

Structure	Level	Pattern	Image	Note
TS25: back skin	Present		S1Ab	Expression in dorsal skin.

Structure	Level	Pattern	Image	Note
TS25: Bowman's capsule of mature renal corpuscle	Strong		S3A
TS25: collecting duct	Strong		S3A

Structure	Level	Pattern	Image	Note
TS25: abdomen	Present		S3B

Structure	Level	Pattern	Image	Note
TS25: neocortex	Present		S3F