Gene Expression Data
Assay Details
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Assay
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Reference:
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J:182791
Brockmann C, et al., Beta- and gamma-cytoplasmic actins are required for meiosis in mouse oocytes. Biol Reprod. 2011 Nov;85(5):1025-39
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6415000
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Gene symbol:
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Actg1
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Gene name:
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actin, gamma, cytoplasmic 1
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Antibody:
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anti-gamma-CYA 2A3
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Detection system:
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rhodamine
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Assay notes:
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Specimens were embedded in polymerized collagen.
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Results
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Specimen
7A:
embryonic day 1.0
(more  )
Note: Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
Specimen
7A:
(close  )
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Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Paraformaldehyde
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Embedding:
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Other - See Notes
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Note:
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Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
Regionally restricted |
7A merged
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7A gamma-CYA
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Expression localized in blastomere cortices. Expression absent or highly reduced in cell-cell contact regions.
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Specimen
7B:
embryonic day 1.5
(more )
Note: 3 cell embryo. Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
Specimen
7B:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 1.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Paraformaldehyde
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Embedding:
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Other - See Notes
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Note:
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3 cell embryo. Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: early embryo |
Present |
Regionally restricted |
7B merged
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7B gamma-CYA
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In 3-cell embryo, expression absent or highly reduced in cell-cell contact regions.
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Specimen
7C:
embryonic day 2.0
(more )
Note: Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
Specimen
7C:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Paraformaldehyde
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Embedding:
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Other - See Notes
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Note:
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Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 4-cell stage embryo |
Present |
Regionally restricted |
7C gamma-CYA
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7C merged
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Expression absent or highly reduced in cell-cell contact regions. Regions of enhanced expression in cortex overlying nuclei.
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Specimen
7D:
embryonic day 2.5
(more )
Note: Before compaction. Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
Specimen
7D:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Paraformaldehyde
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Embedding:
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Other - See Notes
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Note:
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Before compaction. Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 8-cell stage embryo |
Present |
Regionally restricted |
7D gamma-CYA
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7D merged
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Expression absent or highly reduced in cell-cell contact regions.
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Specimen
7E:
embryonic day 2.5
(more )
Note: Compacting embryo. Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
Specimen
7E:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Paraformaldehyde
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Embedding:
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Other - See Notes
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Note:
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Compacting embryo. Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 8-cell stage embryo |
Present |
Regionally restricted |
7E merged
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7E gamma-CYA
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In flattening blastomeres, expression enriched in outer cortex and around apico-lateral area of contact between adjacent cells at site of junction formation. In unpolarized, rounded blastomeres, expression throughout both outer cortex and at cell-cell contact regions.
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Specimen
7F:
embryonic day 3.0
(more )
Note: 8-16 cells. Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
Specimen
7F:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Paraformaldehyde
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Embedding:
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Other - See Notes
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Note:
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8-16 cells. Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Present |
Regionally restricted |
7F gamma-CYA
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7F merged
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In flattening blastomeres, expression enriched in outer cortex and around apico-lateral area of contact between adjacent cells at site of junction formation. In unpolarized, rounded blastomeres, expression throughout both outer cortex and at cell-cell contact regions.
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Specimen
8A:
embryonic day 3.0
(more )
Note: 16-32 cells. Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
Specimen
8A:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Paraformaldehyde
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Embedding:
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Other - See Notes
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Note:
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16-32 cells. Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: compacted morula |
Present |
Regionally restricted |
8A merged
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8A gamma-CYA
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In 16- to 64-cell embryos, expression almost exclusively in apical cortex of outer blastomeres.
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Specimen
8B:
embryonic day 3.5
(more )
Note: 32-64 cells. Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
Specimen
8B:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Paraformaldehyde
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Embedding:
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Other - See Notes
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Note:
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32-64 cells. Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Present |
Regionally restricted |
8B merged
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8B gamma-CYA
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In 16- to 64-cell embryos, expression almost exclusively in apical cortex of outer blastomeres.
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Specimen
8C:
embryonic day 3.5
(more )
Note: 32-64 cells. Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
Specimen
8C:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Paraformaldehyde
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Embedding:
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Other - See Notes
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Note:
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32-64 cells. Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Present |
Regionally restricted |
8C merged
,
8C gamma-CYA
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In 16- to 64-cell embryos, expression almost exclusively in apical cortex of outer blastomeres.
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Specimen
8D:
embryonic day 3.5
(more )
Note: Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
Specimen
8D:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Paraformaldehyde
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|
Embedding:
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Other - See Notes
|
|
Note:
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Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Present |
Regionally restricted |
8D merged
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8D gamma-CYA
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Expression almost exclusively in outer trophoblastic cells.
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Specimen
8E:
embryonic day 3.5
(more )
Note: Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
Specimen
8E:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Paraformaldehyde
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Embedding:
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Other - See Notes
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|
Note:
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Triple labeled: red - Actg1; green - Actb (assay MGI:6414999); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: polar trophectoderm |
Present |
Regionally restricted |
8E merged
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8E gamma-CYA
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Polarized expression in polar trophoblasts. Expression enriched in outer cortex compared to cortex in contact with inner cell mass.
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| TS4: mural trophectoderm |
Present |
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8E merged
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8E gamma-CYA
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Expression did not appear polarized in mural trophoblasts.
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