Gene Expression Data
Assay Details
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Assay
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Reference:
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J:152494
Behesti H, et al., Loss of Tbx2 delays optic vesicle invagination leading to small optic cups. Dev Biol. 2009 Sep 15;333(2):360-72
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6404555
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Gene symbol:
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Gja1
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Gene name:
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gap junction protein, alpha 1
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Results
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Specimen
5G:
embryonic day 10.5
(more  )
Note: Double labeled: green - Gja1; red - propidium iodide.
Specimen
5G:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6 * ICR
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Gja1; red - propidium iodide.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: optic cup outer layer |
Present |
Regionally restricted |
5G
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Expression in dorsal retinal pigmented epithelium.
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Specimen
5H:
embryonic day 10.5; Tbx2tm1Pa/ Tbx2tm1Pa
(more )
Note: Double labeled: green - Gja1; red - propidium iodide.
Specimen
5H:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * ICR
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Mutant Allele(s):
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Tbx2tm1Pa/Tbx2tm1Pa
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Gja1; red - propidium iodide.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: optic cup outer layer |
Strong |
Regionally restricted |
5H
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Higher intensity of expression in dorsal retinal pigmented epithelium in mutant.
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Specimen
5I:
embryonic day 10.5
(more )
Note: Double labeled: green - Gja1; red - propidium iodide.
Specimen
5I:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * ICR
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Gja1; red - propidium iodide.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: optic cup outer layer |
Weak |
Regionally restricted |
5I
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Low levels of expression in nasal retinal pigmented epithelium.
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Specimen
5J:
embryonic day 10.5; Tbx2tm1Pa/ Tbx2tm1Pa
(more )
Note: Double labeled: green - Gja1; red - propidium iodide.
Specimen
5J:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * ICR
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Mutant Allele(s):
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Tbx2tm1Pa/Tbx2tm1Pa
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Gja1; red - propidium iodide.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: optic cup outer layer |
Strong |
Regionally restricted |
5J
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High levels of expression in nasal retinal pigmented epithelium.
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Analysis Tools