Gene Expression Data
Assay Details
Assay
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Reference:
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J:155937
Bell CE, et al., SNAI1 and SNAI2 are asymmetrically expressed at the 2-cell stage and become segregated to the TE in the mouse blastocyst. PLoS One. 2009;4(12):e8530
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6404483
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Gene symbol:
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Snai2
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Gene name:
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snail family zinc finger 2
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Results
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Specimen
2B:
embryonic day 0.5
(more )
Note: Zygote stage embryo. Filamentous actin (red).
Specimen
2B:
(close )
Genetic Background:
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involves: CD-1 * MF1
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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Zygote stage embryo. Filamentous actin (red).
|
Structure |
Level |
Pattern |
Image |
Note |
TS1: 1-cell stage embryo |
Present |
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2B
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Expression is in discrete foci in cortical regions throughout the zygote.
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Specimen
3B Symmetrical:
embryonic day 1.0
(more )
Note: 2-cell stage embryo. Filamentous actin (red).
Specimen
3B Symmetrical:
(close )
Genetic Background:
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involves: CD-1 * MF1
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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2-cell stage embryo. Filamentous actin (red).
|
Structure |
Level |
Pattern |
Image |
Note |
TS2: 2-cell stage embryo |
Present |
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3B Symmetrical
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Proportion of 2-cell stage embryos that had in both cells symmetrical expression (16%), asymmetrical expression (40%), symmetrical and asymmetrical expression (36%), asymmetrical/no detection expression (8%).
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Specimen
3B Asymmetrical:
embryonic day 1.0
(more )
Note: 2-cell stage embryo. Filamentous actin (red).
Specimen
3B Asymmetrical:
(close )
Genetic Background:
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involves: CD-1 * MF1
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Age:
|
embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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2% Paraformaldehyde
|
Note:
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2-cell stage embryo. Filamentous actin (red).
|
Structure |
Level |
Pattern |
Image |
Note |
TS2: 2-cell stage embryo |
Present |
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3B Asymmetrical
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Proportion of 2-cell stage embryos that had in both cells symmetrical expression (16%), asymmetrical expression (40%), symmetrical and asymmetrical expression (36%), asymmetrical/no detection expression (8%).
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Specimen
3B Asym/Symm.:
embryonic day 1.0
(more )
Note: 2-cell stage embryo. Filamentous actin (red).
Specimen
3B Asym/Symm.:
(close )
Genetic Background:
|
involves: CD-1 * MF1
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Age:
|
embryonic day 1.0
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
|
Fixation:
|
2% Paraformaldehyde
|
Note:
|
2-cell stage embryo. Filamentous actin (red).
|
Structure |
Level |
Pattern |
Image |
Note |
TS2: 2-cell stage embryo |
Present |
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3B Asym./Symm.
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Proportion of 2-cell stage embryos that had in both cells symmetrical expression (16%), asymmetrical expression (40%), symmetrical and asymmetrical expression (36%), asymmetrical/no detection expression (8%).
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Specimen
3B Asymmetrical/No detection:
embryonic day 1.0
(more )
Note: 2-cell stage embryo. Filamentous actin (red).
Specimen
3B Asymmetrical/No detection:
(close )
Genetic Background:
|
involves: CD-1 * MF1
|
Age:
|
embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
2% Paraformaldehyde
|
Note:
|
2-cell stage embryo. Filamentous actin (red).
|
Structure |
Level |
Pattern |
Image |
Note |
TS2: 2-cell stage embryo |
Present |
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3B Asymmetrical/No detection
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Proportion of 2-cell stage embryos that had in both cells symmetrical expression (16%), asymmetrical expression (40%), symmetrical and asymmetrical expression (36%), asymmetrical/no detection expression (8%).
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Specimen
4B Asym./No detection:
embryonic day 2.0
(more )
Note: 4-cell stage embryo. Filamentous actin (red).
Specimen
4B Asym./No detection:
(close )
Genetic Background:
|
involves: CD-1 * MF1
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Age:
|
embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
2% Paraformaldehyde
|
Note:
|
4-cell stage embryo. Filamentous actin (red).
|
Specimen
4B Asym/Symm.:
embryonic day 2.0
(more )
Note: 4-cell stage embryo. Filamentous actin (red).
Specimen
4B Asym/Symm.:
(close )
Genetic Background:
|
involves: CD-1 * MF1
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Age:
|
embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
|
Fixation:
|
2% Paraformaldehyde
|
Note:
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4-cell stage embryo. Filamentous actin (red).
|
Structure |
Level |
Pattern |
Image |
Note |
TS3: 4-cell stage embryo |
Present |
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4B Asym./Symm.
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Proportion of 4-cell stage embryos that had asymmetrical and no detection (28%), asymmetrical/symmetrical expression (72%).
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Specimen
5B 8-cell:
embryonic day 2.5
(more )
Note: 8-cell stage embryo. Filamentous actin (red).
Specimen
5B 8-cell:
(close )
Genetic Background:
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involves: CD-1 * MF1
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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8-cell stage embryo. Filamentous actin (red).
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Specimen
5B Compacted:
embryonic day 3.0
(more )
Note: Compacted embryo. Filamentous actin (red).
Specimen
5B Compacted:
(close )
Genetic Background:
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involves: CD-1 * MF1
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
2% Paraformaldehyde
|
Note:
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Compacted embryo. Filamentous actin (red).
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: compacted morula |
Present |
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5B Compacted
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Expression is in the outer cells only and is absent in the inner cells (68% of the blastomeres showed expression).
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Specimen
5B Blastocyst:
embryonic day 3.5
(more )
Note: Blastocyst stage embryo. Filamentous actin (red).
Specimen
5B Blastocyst:
(close )
Genetic Background:
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involves: CD-1 * MF1
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
|
Note:
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Blastocyst stage embryo. Filamentous actin (red).
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