Antibody:	anti-Sox9 (EPR14335; ab185230)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS22: epithelium of urogenital sinus	Absent		S1_100X green , S1_20X , S1_40X green
TS22: mesenchyme of urogenital sinus	Absent		S1_100X green , S1_20X , S1_40X green
TS22: vertebra	Present	Regionally restricted	S1_20X , S1_100X blue , S1_40X blue	Expression was detected in vertebral chondrocytes.

Structure	Level	Pattern	Image	Note
TS23: epithelium of urogenital sinus	Present		1A
TS23: mesenchyme of urogenital sinus	Present		1A
TS23: nephric duct of male	Present		1A
TS23: urethra of male	Present		1A
TS23: urothelium of bladder	Present		1A

Structure	Level	Pattern	Image	Note
TS26: epithelium of urogenital sinus	Present	Regionally restricted	1B	Expression was most prominently in the urogenital sinus epithelium throughout the entire length of prostatic buds.
TS26: mesenchyme of urogenital sinus	Present	Regionally restricted	1B	Expression was detected in some mesenchymal cells in close proximity to the bud surface.

Structure	Level	Pattern	Image	Note
TS24: epithelium of urogenital sinus	Present		2B
TS24: nephric duct	Present		2B
TS24: urethra epithelium	Present		2B
TS24: urothelium of bladder	Present		2B
TS24: mesenchyme of urogenital sinus	Present	Regionally restricted	2B	Expression was detected in mesenchyme adjacent to the urogenital sinus epithelium.

Structure	Level	Pattern	Image	Note
TS24: epithelium of urogenital sinus	Absent		2C
TS24: urethra epithelium	Absent		2C
TS24: urothelium of bladder	Absent		2C
TS24: mesenchyme of urogenital sinus	Present		2C	Normal expression was seen The Wolffian duct epithelium and mesenchyme adjacent to the urogenital sinus epithelium in the conditional knockout mice.
TS24: nephric duct	Present		2C	Normal expression was seen The Wolffian duct epithelium and mesenchyme adjacent to the urogenital sinus epithelium in the conditional knockout mice.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: Sertoli cell	Sertoli cell	Present		2B inset

Structure	Cell Type	Level	Pattern	Image	Note
TS24: Sertoli cell	Sertoli cell	Present		2C inset	Normal expression was seen in Sertoli cells of the conditional knockout.