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Gene Expression Data
Assay Details
Assay
Reference: J:284089 Mattiske D, et al., A novel long non-coding RNA, Leat1, causes reduced anogenital distance and fertility in female mice. Differentiation. 2019 Dec 4;112:1-6
Assay type: RT-PCR
MGI Accession ID: MGI:6404119
Gene symbol: Leat1
Gene name: lncRNA Efnb2 associated transcript 1
Probe: Leat1-pA, Leat1-pB
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Actin and Hprt were used as housekeeping genes for normalization.
Results Image: 1A
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex
E12.5 E12.5 TS20: genital tubercle Present 0.8 µg; total RNA FVB/NJ Female
E13.5 E13.5 TS21: genital tubercle Present 0.8 µg; total RNA FVB/NJ Female
E14.5 E14.5 TS22: genital tubercle Present 0.8 µg; total RNA FVB/NJ Female
E15.5 E15.5 TS23: genital tubercle Present 0.8 µg; total RNA FVB/NJ Female
E16.5 E16.5 TS24: genital tubercle Present 0.8 µg; total RNA FVB/NJ Female
E17.5 E17.5 TS25: genital tubercle Present 0.8 µg; total RNA FVB/NJ Female
E18.5 E18.5 TS26: genital tubercle Ambiguous 0.8 µg; total RNA FVB/NJ Female
Notes:
(a) Expression levels peaked between E13.5 and E14.5, coinciding with sexual differentiation of the genital tubercle. At E15.5, expression decreased before a small peak again at E17.5.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
12/30/2025
MGI 6.24
The Jackson Laboratory