Gene Expression Data
Assay Details
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Assay
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Reference:
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J:149502
Hill P, et al., A SHH-independent regulation of Gli3 is a significant determinant of anteroposterior patterning of the limb bud. Dev Biol. 2009 Apr 15;328(2):506-16
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6404095
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh probe170
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3A:
embryonic day 11.5
(more  )
Specimen
3A:
(close  )
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Genetic Background:
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involves: C3H * C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud mesenchyme |
Present |
Regionally restricted |
3A
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Expression in posterior mesoderm in zone of polarizing activity (ZPA)
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Specimen
3B:
(close )
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Genetic Background:
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involves: C3H * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud mesenchyme |
Present |
Regionally restricted |
3B
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Anterior upregulation of expression. Ectopic anterior expression most prominent in hindlimb bud.
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Specimen
3C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C3H * C57BL/6
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Mutant Allele(s):
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Gli3tm1Urt/Gli3Xt-J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud mesenchyme |
Present |
Regionally restricted |
3C
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Authors report normal posterior expression domain in both fore- and hindlimbs.
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Specimen
E11.5 forelimb not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C3H * C57BL/6
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Mutant Allele(s):
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Gli3tm1Urt/Gli3Xt-J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud mesenchyme |
Present |
Regionally restricted |
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Authors report normal posterior expression domain in both fore- and hindlimbs.
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Analysis Tools
