Gene Expression Data
Assay Details
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Assay
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Reference:
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J:60156
Lee K, et al., Parathyroid hormone-related peptide delays terminal differentiation of chondrocytes during endochondral bone development. Endocrinology. 1996 Nov;137(11):5109-18
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6403324
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Gene symbol:
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Spp1
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Gene name:
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secreted phosphoprotein 1
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Probe:
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osteopontin cDNA6
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Tissue fixed in 10% formalin.
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Results
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Specimen
7Aa/b:
embryonic day 16.5
(more  )
Specimen
7Aa/b:
(close  )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hindlimb long bone |
Present |
Regionally restricted |
7Aa/b
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Expressed in hypertrophic chondrocytes.
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Specimen
7Ad/e:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Pthlhtm1Hmk/Pthlhtm1Hmk
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hindlimb long bone |
Present |
Regionally restricted |
7Ad/e
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Expressed in hypertrophic chondrocytes. There are more of these cells in the mutant than wild type.
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Analysis Tools
