Gene Expression Data
Assay Details
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Assay
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Reference:
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J:60156
Lee K, et al., Parathyroid hormone-related peptide delays terminal differentiation of chondrocytes during endochondral bone development. Endocrinology. 1996 Nov;137(11):5109-18
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6403310
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Gene symbol:
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Pth1r
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Gene name:
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parathyroid hormone 1 receptor
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Probe:
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PTH/PTHrP receptor cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Tissue fixed in 10% formalin.
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Results
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Specimen
3Ad:
embryonic day 16.5
(more  )
Note: proximal end of tibia
Specimen
3Ad:
(close  )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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proximal end of tibia
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: tibia |
Present |
Regionally restricted |
3Ad
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Stongest expression is present in a thin layer of chondrocytes immediate above the hypertrophic zone. It is less abundant in the hypertrophic zone. It is weakly expressed in the proliferating chondroyctes.
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Specimen
3Ba:
embryonic day 16.5
(more )
Note: proximal end of tibia
Specimen
3Ba:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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proximal end of tibia
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: tibia |
Present |
Regionally restricted |
3Ba
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Expression is present in prehypertrophic chondrocytes at the interface between proliferating chondrocytes and hypertrophic chondrocytes; this area is well demarcated in wild type.
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Specimen
3Bd:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Pthlhtm1Hmk/Pthlhtm1Hmk
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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proximal end of tibia
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: tibia |
Present |
Single cells |
3Bd
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The area of expression is less well demarcated in the mutants.
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Analysis Tools
