Nlrp4f Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:284116 Qin D, et al., The subcortical maternal complex protein Nlrp4f is involved in cytoplasmic lattice formation and organelle distribution. Development. 2019 Oct 18;146(20):dev183616
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6402590
Gene symbol:	Nlrp4f
Gene name:	NLR family, pyrin domain containing 4F

Antibody:	Anti-Nlrp4f
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

Specimen 1D GV (Non-extracted): postnatal adult (more

)
Note: Germinal Vesicle (GV) oocytes. Double labeled: green - Nlrp4f; red - Nlrp5 (assay MGI:6402588).

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Present		1D Merge upper , 1D Nlrp4f upper , 1D Enlargement upper	Expression colocalized with Nlrp5 in the subcortex and cytoplasm of mouse oocytes.

2E GV ©

Specimen 2E GV: postnatal adult (more

)
Note: Germinal Vesicle (GV) oocytes.

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Present		2E GV	Expression was localized in the cytoplasm and concentrated at the subcortex.

2E 1-cell ©

Specimen 2E 1-cell: embryonic day 0.5 (more

)

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present		2E 1-cell	Expression was localized in the cytoplasm and concentrated at the subcortex.

2E 2-cell ©

Specimen 2E 2-cell: embryonic day 1.0 (more

)

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present		2E 2-cell	Expression was localized in the cytoplasm and concentrated at the subcortex.

2E Morula ©

Specimen 2E Morula: embryonic day 3.0 (more

)

Structure	Level	Pattern	Image	Note
TS4: compacted morula	Present		2E Morula	Expression was localized in the cytoplasm and concentrated at the subcortex.

Specimen 2E Blastocyst: embryonic day 3.5 (more

)

Structure	Level	Pattern	Image	Note
TS5: blastocyst	Present		2E Blastocyst	Expression was localized in the cytoplasm and concentrated at the subcortex.

Specimen S2D Nlrp4f+/+: postnatal adult (more

)
Note: Top: antibody alone. Bottom: Antibody + Hoechst. Germinal Vesicle (GV) oocytes.

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Present		S2D Nlrp4f+/+

Specimen S2D Nlrp4f-/-: postnatal adult; Nlrp4f^em1Lil/Nlrp4f^em1Lil (more

)
Note: Top: antibody alone. Bottom: Antibody + Hoechst. Germinal Vesicle (GV) oocytes.

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Absent		S2D Nlrp4f-/-

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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