Atg9a Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:284681 Ivankovic D, et al., Axonal autophagosome maturation defect through failure of ATG9A sorting underpins pathology in AP-4 deficiency syndrome. Autophagy. 2020 Mar;16(3):391-407
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6402274
Gene symbol:	Atg9a
Gene name:	autophagy related 9A

Antibody:	Anti-Atg9a [EPR2450(2)] (ab108338)
Detection system:	Secondary antibody coupled to Alexa Fluor
Assay notes:	Three antibodies were described: MGI:6402268, MGI:6402270, and MGI:6402272. The antibody used in this assay was not specified.

Results

2d WT ATG9A ©

2d WT NF200 ©

2e WT ATG9A ©

2e WT NF200 ©

2f WT ATG9A ©

2f WT NF200 ©

Specimen 2d WT: postnatal month 1 (more

)
Note: Double labeled: red - Atg9a; blue - Nefh (assay MGI:6402249).

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		2d WT NF200 , 2e WT NF200 , 2d WT ATG9A , 2e WT ATG9A
TS28: hippocampus CA1	Present		2f WT NF200 , 2d WT ATG9A , 2f WT ATG9A , 2d WT NF200

2d KO ATG9A ©

2d KO NF200 ©

2e KO ATG9A ©

2e KO NF200 ©

2f KO ATG9A ©

2f KO NF200 ©

Specimen 2d KO: postnatal month 1; Ap4e1^{tm1b(KOMP)Wtsi}/Ap4e1^{tm1b(KOMP)Wtsi} (more

)
Note: Double labeled: red - Atg9a; blue - Nefh (assay MGI:6402249).

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		2e KO NF200 , 2d KO NF200 , 2d KO ATG9A , 2f KO ATG9A , 2e KO ATG9A , 2f KO NF200	A marked increased in the immunoreactivity in the mutant brain appeared to accumulate in neuronal cell layers of the cortex and CA1 regions.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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