Gene Expression Data
Assay Details
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Assay
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Reference:
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J:242062
Wolfe AD, et al., STELLA collaborates in distinct mesendodermal cell subpopulations at the fetal-placental interface in the mouse gastrula. Dev Biol. 2017 May 01;425(1):44-57
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6401474
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Gene symbol:
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Mixl1
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Gene name:
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Mix paired-like homeobox
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Antibody:
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anti-MIXl1
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Detection system:
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Secondary antibody coupled to DyLight 549
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Results
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Specimen
5B/D:
embryonic day 7.5
(more  )
Note: Early headfold stage. Triple labeled: green - Mixl1; red - T (assay MGI:6401468); blue - DAPI.
Specimen
5B/D:
(close  )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Early headfold stage. Triple labeled: green - Mixl1; red - T (assay MGI:6401468); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: allantois mesoderm |
Present |
Regionally restricted |
5B
,
5D
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Co-expression with T in all Mixl1-expressing cells within posterior primitive streak and associated posterior visceral endoderm. No expression in some T-expressing cells.
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| TS11: posterior visceral endoderm |
Present |
Regionally restricted |
5B
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5D
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Co-expression with T in all Mixl1-expressing cells within posterior primitive streak and associated posterior visceral endoderm. No expression in some T-expressing cells.
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Specimen
8s stage not shown:
embryonic day 8.5
(more )
Note: 8 somite stage.
Specimen
8s stage not shown:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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8 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: allantois mesenchyme |
Present |
Regionally restricted |
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In late hindgut stages, cells co-expressing T persisted in ACD/presumptive tailbud mesoderm.
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| TS13: notochord |
Present |
Regionally restricted |
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Cells co-expressing T limited to posterior edge of notochord.
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| TS13: hindgut diverticulum |
Present |
Regionally restricted |
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Cells co-expressing T predominated in hindgut's dorsal component during hindgut elongation.
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Specimen
5E/H:
embryonic day 7.0
(more )
Note: Early bud stage. Triple labeled: green - Mixl1; red - Stella (Dppa3 assay MGI:6401471); blue - DAPI.
Specimen
5E/H:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 7.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Early bud stage. Triple labeled: green - Mixl1; red - Stella (Dppa3 assay MGI:6401471); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: allantois mesoderm |
Present |
Regionally restricted |
5H
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5F
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No co-expression with Stella (Dppa3) in extraembryonic primitive streak (XPS).
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