Gene Expression Data
Assay Details
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Assay
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Reference:
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J:242062
Wolfe AD, et al., STELLA collaborates in distinct mesendodermal cell subpopulations at the fetal-placental interface in the mouse gastrula. Dev Biol. 2017 May 01;425(1):44-57
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6401473
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Gene symbol:
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Pou5f1
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Gene name:
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POU domain, class 5, transcription factor 1
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Antibody:
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anti-Oct4 (N-19; sc-8628)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
3M:
embryonic day 8.0
(more  )
Note: 7 somite stage.
Specimen
3M:
(close  )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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7 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: splanchnopleure |
Strong |
Punctate |
3M
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Strong punctate expression in cells with large, prominent nuclei localized in splanchnopleure just anterior to site of splanchnpleure closure to form hindgut.
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| TS12: future spinal cord neural fold |
Weak |
Regionally restricted |
3M
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Fainter expression in cells with smaller nuclei throughout neural folds and subjacent mesoderm.
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| TS12: trunk mesenchyme |
Weak |
Regionally restricted |
3M
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Fainter expression in cells with smaller nuclei throughout neural folds and subjacent mesoderm.
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Specimen
3N:
embryonic day 8.0
(more )
Note: 7 somite stage.
Specimen
3N:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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7 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: primitive hindgut |
Strong |
Punctate |
3N
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Strong punctate expression in cells with large, prominent nuclei at site of hindgut closure.
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| TS12: future spinal cord neural fold |
Weak |
Regionally restricted |
3N
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Fainter expression in cells with smaller nuclei throughout neural folds and subjacent mesoderm.
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| TS12: trunk mesenchyme |
Weak |
Regionally restricted |
3N
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Fainter expression in cells with smaller nuclei throughout neural folds and subjacent mesoderm.
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Analysis Tools
